Restriction map for
pF1KE2522
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BcgI BciVI BclI BfrI
BlnI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HgaI
HindIII HpaI KpnI MfeI MluI
MseI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SbfI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccIII 1728 TCCGGA T^CCGGA
Alw44I 2317 GTGCAC G^TGCAC
ApaLI 2317 GTGCAC G^TGCAC
Asp700I 2700 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaBI 141 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1872 GGTCTC GGTCTC(1/5)
BsaWI 1728 TCCGGA [AT]^CCGG[AT]
BseAI 1728 TCCGGA T^CCGGA
BspEI 1728 TCCGGA T^CCGGA
BsrDI 310 GCAATG GCAATG(2/0)
BstXI 609 CCAGCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
HincII 1198 GTCGAC GT[CT]^[GA]AC
HindII 1198 GTCGAC GT[CT]^[GA]AC
MamI 141 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1728 TCCGGA T^CCGGA
NheI 1656 GCTAGC G^CTAGC
NspI 1787 ACATGC [GA]CATG^[CT]
PflFI 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 1198 GTCGAC G^TCGAC
SexAI 1100 ACCAGGT A^CC[AT]GGT
SfiI 2295 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2256 CCCGGG CCC^GGG
TfiI 2700 GAATC G^A[AT]TC
Tth111I 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2256 CCCGGG C^CCGGG
XmaI 2256 CCCGGG C^CCGGG
XmnI 2700 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccI 1198 GTCGAC GT^[AC][GT]AC
1639 GTCTAC
AcyI 1285 GGCGCC G[GA]^CG[CT]C
2153 GGCGCC
AfaI 1863 GTAC GT^AC
2203 GTAC
AocI 475 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
ApaI 61 GGGCCC GGGCC^C
214 GGGCCC
AvaI 1693 CTCGGG C^[CT]CG[GA]G
2256 CCCGGG
AviII 1541 TGCGCA TGC^GCA
1568 TGCGCA
BalI 1112 TGGCCA TGG^CCA
2303 TGGCCA
BamHI 343 GGATCC G^GATCC
821 GGATCC
BbeI 1285 GGCGCC GGCGC^C
2153 GGCGCC
BbrPI 1393 CACGTG CAC^GTG
1669 CACGTG
BbsI 646 GTCTTC GAAGAC(2/6)
1381 GTCTTC
BbvCI 800 CCTCAGC CCTCAGC(-5/-2)
1173 CCTCAGC
BpuAI 646 GTCTTC GAAGAC(2/6)
1381 GTCTTC
BsaAI 1393 CACGTG [CT]AC^GT[GA]
1669 CACGTG
BsaHI 1285 GGCGCC G[GA]^CG[CT]C
2153 GGCGCC
BsoBI 1693 CTCGGG C^[CT]CG[GA]G
2256 CCCGGG
BssSI 1489 CACGAG CACGAG(-5/-1)
1940 CTCGTG
BstEII 1698 GGTCACC G^GT[ACGT]ACC
2724 GGTGACC
BstPI 1698 GGTCACC G^GT[ACGT]ACC
2724 GGTGACC
Bsu36I 475 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
Eco81I 475 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
EcoO65I 1698 GGTCACC G^GT[ACGT]ACC
2724 GGTGACC
FspI 1541 TGCGCA TGC^GCA
1568 TGCGCA
Hin1I 1285 GGCGCC G[GA]^CG[CT]C
2153 GGCGCC
KasI 1285 GGCGCC G^GCGCC
2153 GGCGCC
MluNI 1112 TGGCCA TGG^CCA
2303 TGGCCA
MscI 1112 TGGCCA TGG^CCA
2303 TGGCCA
NaeI 865 GCCGGC GCC^GGC
1131 GCCGGC
NarI 1285 GGCGCC GG^CGCC
2153 GGCGCC
NgoMIV 865 GCCGGC G^CCGGC
1131 GCCGGC
PflMI 567 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2451 CCAGAAGCTGG
PmaCI 1393 CACGTG CAC^GTG
1669 CACGTG
PmlI 1393 CACGTG CAC^GTG
1669 CACGTG
RsaI 1863 GTAC GT^AC
2203 GTAC
SacI 972 GAGCTC GAGCT^C
1888 GAGCTC
SfoI 1285 GGCGCC GGC^GCC
2153 GGCGCC
Tsp509I 662 AATT ^AATT
2406 AATT
Van91I 567 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2451 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII