Restriction map for
pF1KE8000
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BbrPI BcgI BciVI
BfaI BfrI BglI BlnI BsaAI
BsiEI BsiWI BsmBI BsoBI BspDI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
HindIII HpaI KpnI KspI MaeI
MaeII MaeIII MfeI MluI MluNI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 244 GGCGCC G[GA]^CG[CT]C
AfaI 43 GTAC GT^AC
AhdI 55 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 55 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 244 GGCGCC GGCGC^C
BbvCI 222 GCTGAGG CCTCAGC(-5/-2)
BsaBI 444 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 244 GGCGCC G[GA]^CG[CT]C
BsgI 418 GTGCAG GTGCAG(16/14)
BsmI 1132 GAATGC GAATGC(1/-1)
Bsp1407I 42 TGTACA T^GTACA
BspHI 164 TCATGA T^CATGA
BspMI 934 GCAGGT ACCTGC(4/8)
BsrBI 312 GAGCGG CCGCTC(-3/-3)
BsrFI 905 GCCGGC [GA]^CCGG[CT]
BsrGI 42 TGTACA T^GTACA
BstAPI 536 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 905 GCCGGC [GA]^CCGG[CT]
DraIII 1075 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 350 CCGTGG C^C[GA][CT]GG
EaeI 903 TGGCCG [CT]^GGCC[GA]
Eam1105I 55 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1042 CTCTTC CTCTTC(1/4)
HaeII 244 GGCGCC [GA]GCGC^[CT]
HgaI 466 GACGC GACGC(5/10)
Hin1I 244 GGCGCC G[GA]^CG[CT]C
KasI 244 GGCGCC G^GCGCC
Ksp632I 1042 CTCTTC CTCTTC(1/4)
MamI 444 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 905 GCCGGC GCC^GGC
NarI 244 GGCGCC GG^CGCC
NgoMIV 905 GCCGGC G^CCGGC
PpuMI 108 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 164 TCATGA T^CATGA
RsaI 43 GTAC GT^AC
SacI 1195 GAGCTC GAGCT^C
SfoI 244 GGCGCC GGC^GCC
SspBI 42 TGTACA T^GTACA
StuI 284 AGGCCT AGG^CCT
TfiI 860 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 879 CGCG CG^CG
1078 CGCG
AccIII 236 TCCGGA T^CCGGA
605 TCCGGA
AcsI 510 GAATTT [GA]^AATT[CT]
973 AAATTC
ApoI 510 GAATTT [GA]^AATT[CT]
973 AAATTC
AspHI 618 GTGCTC G[AT]GC[AT]^C
1195 GAGCTC
AviII 296 TGCGCA TGC^GCA
1235 TGCGCA
BanI 227 GGTGCC G^G[CT][GA]CC
244 GGCGCC
BanII 961 GAGCCC G[GA]GC[CT]^C
1195 GAGCTC
BbsI 264 GAAGAC GAAGAC(2/6)
925 GAAGAC
BlpI 531 GCTTAGC GC^T[ACGT]AGC
1213 GCTCAGC
BpmI 307 CTGGAG CTGGAG(16/14)
958 CTGGAG
Bpu1102I 531 GCTTAGC GC^T[ACGT]AGC
1213 GCTCAGC
BpuAI 264 GAAGAC GAAGAC(2/6)
925 GAAGAC
BsaI 9 GAGACC GGTCTC(1/5)
630 GGTCTC
BsaWI 236 TCCGGA [AT]^CCGG[AT]
605 TCCGGA
BseAI 236 TCCGGA T^CCGGA
605 TCCGGA
BsiHKAI 618 GTGCTC G[AT]GC[AT]^C
1195 GAGCTC
BspEI 236 TCCGGA T^CCGGA
605 TCCGGA
BstUI 879 CGCG CG^CG
1078 CGCG
CelII 531 GCTTAGC GC^T[ACGT]AGC
1213 GCTCAGC
DraII 108 AGGTCCC [GA]G^G[ACGT]CC[CT]
566 AGGCCCC
EcoO109I 108 AGGTCCC [GA]G^G[ACGT]CC[CT]
566 AGGCCCC
FspI 296 TGCGCA TGC^GCA
1235 TGCGCA
MroI 236 TCCGGA T^CCGGA
605 TCCGGA
MslI 154 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1179 CATGGCTATG
MvnI 879 CGCG CG^CG
1078 CGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AflIII AluI AlwNI AvaII
BbvI BclI BcnI BglII BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoNI EcoRII
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I
XhoII