Restriction map for
pF1KB9838
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HinfI HpaI KpnI Ksp632I MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwI 342 GGATC GGATC(4/5)
AlwNI 198 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 297 GGGCCC GGGCC^C
BanII 297 GGGCCC G[GA]GC[CT]^C
BbrPI 655 CACGTG CAC^GTG
BlpI 162 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC
BsaAI 655 CACGTG [CT]AC^GT[GA]
BsgI 823 GTGCAG GTGCAG(16/14)
BsmAI 261 GTCTC GTCTC(1/5)
BsmBI 260 CGTCTC CGTCTC(1/5)
Bsp1407I 923 TGTACA T^GTACA
BsrGI 923 TGTACA T^GTACA
BsrI 350 ACTGG ACTGG(1/-1)
BstAPI 167 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 612 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 342 GGATCT [GA]^GATC[CT]
CelII 162 GCTCAGC GC^T[ACGT]AGC
DpnII 343 GATC ^GATC
DraIII 652 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 921 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
MboI 343 GATC ^GATC
MflI 342 GGATCT [GA]^GATC[CT]
NcoI 618 CCATGG C^CATGG
NdeII 343 GATC ^GATC
NotI 472 GCGGCCGC GC^GGCCGC
PmaCI 655 CACGTG CAC^GTG
PmlI 655 CACGTG CAC^GTG
PpuMI 917 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 343 GATC ^GATC
SfaNI 369 GCATC GCATC(5/9)
SfiI 952 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 222 CACCGGCG C[GA]^CCGG[CT]G
SspBI 923 TGTACA T^GTACA
XhoII 342 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 116 AGCGCT AGC^GCT
671 AGCGCT
AvaII 918 GGACC G^G[AT]CC
1018 GGTCC
BalI 47 TGGCCA TGG^CCA
905 TGGCCA
BsiEI 473 CGGCCG CG[GA][CT]^CG
1040 CGGCCG
BstF5I 753 CATCC GGATG(2/0)
353 GGATG
DdeI 163 CTCAG C^T[ACGT]AG
263 CTCAG
EagI 473 CGGCCG C^GGCCG
1040 CGGCCG
EclXI 473 CGGCCG C^GGCCG
1040 CGGCCG
Eco47III 116 AGCGCT AGC^GCT
671 AGCGCT
Eco52I 473 CGGCCG C^GGCCG
1040 CGGCCG
EcoT14I 618 CCATGG C^C[AT][AT]GG
1031 CCAAGG
FokI 753 CATCC GGATG(9/13)
353 GGATG
HphI 90 GGTGA GGTGA(8/7)
372 TCACC
MaeII 59 ACGT A^CGT
656 ACGT
MboII 93 GAAGA GAAGA(8/7)
1049 TCTTC
MluNI 47 TGGCCA TGG^CCA
905 TGGCCA
MscI 47 TGGCCA TGG^CCA
905 TGGCCA
NaeI 330 GCCGGC GCC^GGC
544 GCCGGC
NgoMIV 330 GCCGGC G^CCGGC
544 GCCGGC
PvuII 688 CAGCTG CAG^CTG
880 CAGCTG
SmlI 760 CTCAAG C^T[CT][GA]AG
781 CTCAAG
StyI 618 CCATGG C^C[AT][AT]GG
1031 CCAAGG
XcmI 35 CCAGCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
609 CCACCACCACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI KspI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
RsaI SacII Sau96I ScrFI SfoI
SmaI TaqI TseI TthHB8I XmaCI
XmaI