Restriction map for
pF1KE2430
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI Asp718I AspEI AspI AviII
BamHI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaI BseAI BsiWI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NcoI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 175 GACGTC GACGT^C
AflIII 1124 ACGTGT A^C[GA][CT]GT
Alw44I 270 GTGCAC G^TGCAC
AlwNI 262 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 742 CCTGAGG CC^T[ACGT]AGG
ApaI 1587 GGGCCC GGGCC^C
ApaLI 270 GTGCAC G^TGCAC
AseI 546 ATTAAT AT^TAAT
AsnI 546 ATTAAT AT^TAAT
Asp700I 460 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 162 CCTAGG C^CTAGG
BalI 1079 TGGCCA TGG^CCA
BbsI 142 GTCTTC GAAGAC(2/6)
BcgI 597 CGAAGAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 163 CTAG C^TAG
BlnI 162 CCTAGG C^CTAGG
BpuAI 142 GTCTTC GAAGAC(2/6)
BsaBI 1426 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 452 ACCGGA [AT]^CCGG[AT]
BsgI 1544 CTGCAC GTGCAG(16/14)
BsmAI 1362 GTCTC GTCTC(1/5)
BsmBI 1361 CGTCTC CGTCTC(1/5)
BsmI 460 GAATGC GAATGC(1/-1)
Bsp1407I 541 TGTACA T^GTACA
BspHI 1070 TCATGA T^CATGA
BspMI 406 GCAGGT ACCTGC(4/8)
BsrGI 541 TGTACA T^GTACA
BstYI 311 AGATCC [GA]^GATC[CT]
Bsu36I 742 CCTGAGG CC^T[ACGT]AGG
DraI 849 TTTAAA TTT^AAA
DraIII 569 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 186 CCACGG C^C[GA][CT]GG
Eco81I 742 CCTGAGG CC^T[ACGT]AGG
EcoRV 500 GATATC GAT^ATC
MaeI 163 CTAG C^TAG
MamI 1426 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 311 AGATCC [GA]^GATC[CT]
MluNI 1079 TGGCCA TGG^CCA
MscI 1079 TGGCCA TGG^CCA
PflMI 1333 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 546 ATTAAT AT^TAAT
PstI 842 CTGCAG CTGCA^G
RcaI 1070 TCATGA T^CATGA
SacI 1036 GAGCTC GAGCT^C
SexAI 137 ACCTGGT A^CC[AT]GGT
SspBI 541 TGTACA T^GTACA
Van91I 1333 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 311 AGATCC [GA]^GATC[CT]
XmnI 460 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 312 GATCC GGATC(4/5)
1564 GATCC
AvaI 29 CCCGAG C^[CT]CG[GA]G
108 CTCGGG
BbeI 96 GGCGCC GGCGC^C
112 GGCGCC
BpmI 712 CTCCAG CTGGAG(16/14)
1232 CTCCAG
BseRI 55 CTCCTC GAGGAG(10/8)
1318 CTCCTC
BsiEI 127 CGGCCG CG[GA][CT]^CG
227 CGGCCG
BsoBI 29 CCCGAG C^[CT]CG[GA]G
108 CTCGGG
BsrFI 125 GCCGGC [GA]^CCGG[CT]
229 GCCGGC
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
229 GCCGGC
EagI 127 CGGCCG C^GGCCG
227 CGGCCG
EclXI 127 CGGCCG C^GGCCG
227 CGGCCG
Eco52I 127 CGGCCG C^GGCCG
227 CGGCCG
HaeII 96 GGCGCC [GA]GCGC^[CT]
112 GGCGCC
HinfI 1201 GACTC G^A[ACGT]TC
1246 GACTC
HphI 644 TCACC GGTGA(8/7)
1207 TCACC
KasI 96 GGCGCC G^GCGCC
112 GGCGCC
MaeII 176 ACGT A^CGT
1124 ACGT
NaeI 125 GCCGGC GCC^GGC
229 GCCGGC
NarI 96 GGCGCC GG^CGCC
112 GGCGCC
NdeI 421 CATATG CA^TATG
761 CATATG
NgoMIV 125 GCCGGC G^CCGGC
229 GCCGGC
PleI 1201 GACTC GAGTC(4/5)
1246 GACTC
PvuII 289 CAGCTG CAG^CTG
357 CAGCTG
SfaNI 197 GCATC GCATC(5/9)
789 GCATC
SfoI 96 GGCGCC GGC^GCC
112 GGCGCC
SspI 1180 AATATT AAT^ATT
1262 AATATT
XcmI 288 CCAGCTGCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1217 CCAGGAACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I