Restriction map for
pF1KE2112
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI BamHI BbrPI BbvCI BcgI
BfrI BsaBI BsaI BsaWI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoT22I FseI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2593 GTAGAC GT^[AC][GT]AC
AflIII 2536 ACACGT A^C[GA][CT]GT
Alw44I 745 GTGCAC G^TGCAC
AocI 1239 CCTAAGG CC^T[ACGT]AGG
Aor51HI 341 AGCGCT AGC^GCT
ApaI 288 GGGCCC GGGCC^C
ApaLI 745 GTGCAC G^TGCAC
AseI 2227 ATTAAT AT^TAAT
AsnI 2227 ATTAAT AT^TAAT
AvaI 855 CTCGAG C^[CT]CG[GA]G
AvrII 1600 CCTAGG C^CTAGG
BciVI 2158 GGATAC GTATCC(6/5)
BclI 2133 TGATCA T^GATCA
BlnI 1600 CCTAGG C^CTAGG
BsaAI 559 TACGTG [CT]AC^GT[GA]
BsiEI 781 CGGCCG CG[GA][CT]^CG
BsmFI 809 GGGAC GGGAC(10/14)
BsmI 2519 GCATTC GAATGC(1/-1)
BsoBI 855 CTCGAG C^[CT]CG[GA]G
BsrBI 778 GAGCGG CCGCTC(-3/-3)
BsrFI 269 GCCGGC [GA]^CCGG[CT]
BssHII 517 GCGCGC G^CGCGC
BstBI 1441 TTCGAA TT^CGAA
BstXI 707 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1239 CCTAAGG CC^T[ACGT]AGG
Cfr10I 269 GCCGGC [GA]^CCGG[CT]
DrdI 1723 GACAGTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 781 CGGCCG C^GGCCG
EclXI 781 CGGCCG C^GGCCG
Eco47III 341 AGCGCT AGC^GCT
Eco52I 781 CGGCCG C^GGCCG
Eco81I 1239 CCTAAGG CC^T[ACGT]AGG
EcoNI 1430 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2647 GATATC GAT^ATC
FbaI 2133 TGATCA T^GATCA
HindIII 1127 AAGCTT A^AGCTT
HpaI 2389 GTTAAC GTT^AAC
KspI 312 CCGCGG CCGC^GG
NaeI 269 GCCGGC GCC^GGC
NcoI 218 CCATGG C^CATGG
NgoMIV 269 GCCGGC G^CCGGC
NotI 780 GCGGCCGC GC^GGCCGC
NspV 1441 TTCGAA TT^CGAA
PaeR7I 855 CTCGAG C^TCGAG
PshBI 2227 ATTAAT AT^TAAT
PvuII 454 CAGCTG CAG^CTG
SacII 312 CCGCGG CCGC^GG
SfiI 954 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1441 TTCGAA TT^CGAA
SphI 2747 GCATGC GCATG^C
XhoI 855 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 1349 AAATTC [GA]^AATT[CT]
2140 AAATTC
AlwNI 637 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1472 CAGTTACTG
ApoI 1349 AAATTC [GA]^AATT[CT]
2140 AAATTC
Asp700I 975 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGCCATTC
AviII 383 TGCGCA TGC^GCA
563 TGCGCA
BbsI 67 GAAGAC GAAGAC(2/6)
2677 GAAGAC
BlpI 939 GCTCAGC GC^T[ACGT]AGC
1860 GCTCAGC
Bpu1102I 939 GCTCAGC GC^T[ACGT]AGC
1860 GCTCAGC
BpuAI 67 GAAGAC GAAGAC(2/6)
2677 GAAGAC
BsmBI 613 CGTCTC CGTCTC(1/5)
240 GAGACG
BsrDI 1659 CATTGC GCAATG(2/0)
1011 GCAATG
BstAPI 636 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
747 GCACATGCTGC
BstEII 39 GGTGACC G^GT[ACGT]ACC
483 GGTGACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
483 GGTGACC
CelII 939 GCTCAGC GC^T[ACGT]AGC
1860 GCTCAGC
EarI 2470 CTCTTC CTCTTC(1/4)
1060 GAAGAG
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
483 GGTGACC
FspI 383 TGCGCA TGC^GCA
563 TGCGCA
Ksp632I 2470 CTCTTC CTCTTC(1/4)
1060 GAAGAG
MslI 246 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
372 CATCCCAGTG
PpuMI 914 AGGACCC [GA]G^G[AT]CC[CT]
1596 AGGACCT
SacI 937 GAGCTC GAGCT^C
1582 GAGCTC
SspI 2091 AATATT AAT^ATT
2152 AATATT
StuI 1236 AGGCCT AGG^CCT
1836 AGGCCT
XmnI 975 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGCCATTC
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BglII BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII