Restriction map for
pF1KE3145
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BmyI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 140 GTAC GT^AC
AlwI 397 GATCC GGATC(4/5)
AspI 691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 530 CTCGAG C^[CT]CG[GA]G
BbsI 706 GAAGAC GAAGAC(2/6)
BbvCI 370 GCTGAGG CCTCAGC(-5/-2)
BglII 613 AGATCT A^GATCT
BlpI 79 GCTGAGC GC^T[ACGT]AGC
BpmI 55 CTGGAG CTGGAG(16/14)
Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC
BpuAI 706 GAAGAC GAAGAC(2/6)
BsoBI 530 CTCGAG C^[CT]CG[GA]G
BssSI 700 CTCGTG CACGAG(-5/-1)
BstAPI 676 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 831 GGTAACC G^GT[ACGT]ACC
BstPI 831 GGTAACC G^GT[ACGT]ACC
BstXI 168 CCATAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 412 GCCTGC GC[ACGT]^[ACGT]GC
CelII 79 GCTGAGC GC^T[ACGT]AGC
DraIII 677 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 831 GGTAACC G^GT[ACGT]ACC
EcoRI 404 GAATTC G^AATTC
EcoRV 814 GATATC GAT^ATC
HgaI 1009 GACGC GACGC(5/10)
HindIII 64 AAGCTT A^AGCTT
HphI 741 TCACC GGTGA(8/7)
MslI 169 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 676 GCACTTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 655 ACATGC [GA]CATG^[CT]
PaeR7I 530 CTCGAG C^TCGAG
PflFI 691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 140 GTAC GT^AC
ScaI 139 AGTACT AGT^ACT
SmlI 530 CTCGAG C^T[CT][GA]AG
StuI 410 AGGCCT AGG^CCT
TaqI 531 TCGA T^CGA
Tth111I 691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 531 TCGA T^CGA
XbaI 921 TCTAGA T^CTAGA
XhoI 530 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 488 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
753 CAGTATCTG
Asp700I 11 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAAAGCCTTC
AvaII 306 GGACC G^G[AT]CC
696 GGTCC
BfaI 244 CTAG C^TAG
922 CTAG
BsgI 674 CTGCAC GTGCAG(16/14)
299 GTGCAG
BsmI 193 GAATGC GAATGC(1/-1)
725 GAATGC
BsrDI 230 CATTGC GCAATG(2/0)
325 GCAATG
BssKI 339 CCTGG ^CC[ACGT]GG
400 CCAGG
BstNI 339 CCTGG CC^[AT]GG
400 CCAGG
BstYI 396 AGATCC [GA]^GATC[CT]
613 AGATCT
DraI 571 TTTAAA TTT^AAA
933 TTTAAA
EcoRII 339 CCTGG ^CC[AT]GG
400 CCAGG
EcoT14I 270 CCTTGG C^C[AT][AT]GG
836 CCAAGG
HaeIII 411 GGCC GG^CC
875 GGCC
MaeI 244 CTAG C^TAG
922 CTAG
MflI 396 AGATCC [GA]^GATC[CT]
613 AGATCT
MvaI 339 CCTGG CC^[AT]GG
400 CCAGG
PleI 448 GACTC GAGTC(4/5)
31 GAGTC
ScrFI 339 CCTGG CC^[ACGT]GG
400 CCAGG
StyI 270 CCTTGG C^C[AT][AT]GG
836 CCAAGG
XhoII 396 AGATCC [GA]^GATC[CT]
613 AGATCT
XmnI 11 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsrI BstF5I Cfr13I DdeI DpnII
EcoT22I Fnu4HI FokI HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NsiI Sau3AI Sau96I
SfaNI TfiI Tru9I TseI Tsp509I
TspRI