Restriction map for
pF1KE2202
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I FauI
FseI FspI HindIII KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 2786 TCCGGA T^CCGGA
AciI 847 GCGG CCGC(-3/-1)
AcyI 1574 GGCGTC G[GA]^CG[CT]C
Aor51HI 1493 AGCGCT AGC^GCT
AspHI 1008 GAGCAC G[AT]GC[AT]^C
BalI 2655 TGGCCA TGG^CCA
BbrPI 68 CACGTG CAC^GTG
BbsI 1743 GAAGAC GAAGAC(2/6)
BclI 426 TGATCA T^GATCA
BcnI 999 CCCGG CC^[GC]GG
BpuAI 1743 GAAGAC GAAGAC(2/6)
BsaAI 68 CACGTG [CT]AC^GT[GA]
BsaBI 2533 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1574 GGCGTC G[GA]^CG[CT]C
BsaI 974 GGTCTC GGTCTC(1/5)
BseAI 2786 TCCGGA T^CCGGA
BsiHKAI 1008 GAGCAC G[AT]GC[AT]^C
BsmI 1215 GAATGC GAATGC(1/-1)
BspEI 2786 TCCGGA T^CCGGA
BspHI 1815 TCATGA T^CATGA
BspLU11I 1984 ACATGT A^CATGT
BstAPI 224 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2689 GGTGACC G^GT[ACGT]ACC
BstPI 2689 GGTGACC G^GT[ACGT]ACC
BstXI 1579 CCATCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 843 GCATGC GC[ACGT]^[ACGT]GC
CfoI 1494 GCGC GCG^C
DraI 1782 TTTAAA TTT^AAA
DraII 2483 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2655 TGGCCA [CT]^GGCC[GA]
Eco47III 1493 AGCGCT AGC^GCT
EcoO109I 2483 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2689 GGTGACC G^GT[ACGT]ACC
EcoT22I 1654 ATGCAT ATGCA^T
FbaI 426 TGATCA T^GATCA
HaeII 1493 AGCGCT [GA]GCGC^[CT]
HhaI 1494 GCGC GCG^C
Hin1I 1574 GGCGTC G[GA]^CG[CT]C
HinP1I 1494 GCGC G^CGC
HpaI 2680 GTTAAC GTT^AAC
MamI 2533 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2655 TGGCCA TGG^CCA
MroI 2786 TCCGGA T^CCGGA
MscI 2655 TGGCCA TGG^CCA
MslI 430 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1286 CAGCTG C[AC]G^C[GT]G
NciI 999 CCCGG CC^[GC]GG
NlaIV 2484 GGACCC GG[ACGT]^[ACGT]CC
NsiI 1654 ATGCAT ATGCA^T
PmaCI 68 CACGTG CAC^GTG
PmlI 68 CACGTG CAC^GTG
PpuMI 2483 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1286 CAGCTG CAG^CTG
RcaI 1815 TCATGA T^CATGA
SalI 53 GTCGAC G^TCGAC
SapI 1387 GAAGAGC GCTCTTC(1/4)
SpeI 2181 ACTAGT A^CTAGT
SphI 843 GCATGC GCATG^C
XcmI 1579 CCATCTGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 1984 ACATGT A^C[GA][CT]GT
2227 ACACGT
Asp700I 1562 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2797 GAAAGTGTTC
BciVI 1143 GGATAC GTATCC(6/5)
2586 GGATAC
BsaJI 62 CCAGGG C^C[ACGT][ACGT]GG
998 CCCCGG
BsaWI 10 TCCGGT [AT]^CCGG[AT]
2786 TCCGGA
BseRI 1606 GAGGAG GAGGAG(10/8)
2255 GAGGAG
BsgI 1013 CTGCAC GTGCAG(16/14)
1943 CTGCAC
BsmFI 1308 GGGAC GGGAC(10/14)
830 GTCCC
Bst1107I 2444 GTATAC GTA^TAC
2572 GTATAC
BstZ17I 2444 GTATAC GTA^TAC
2572 GTATAC
HgaI 7 GCGTC GACGC(5/10)
1575 GCGTC
MaeII 69 ACGT A^CGT
2229 ACGT
SfcI 36 CTACAG C^T[GA][CT]AG
289 CTACAG
SspI 1708 AATATT AAT^ATT
2356 AATATT
XbaI 1231 TCTAGA T^CTAGA
1900 TCTAGA
XmnI 1562 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2797 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BglII
BmyI BpmI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NspI
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII