Restriction map for
pF1KE1861
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsoBI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeIII MamI MfeI MflI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI TfiI Tsp45I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 981 GACGTC GACGT^C
AccI 898 GTCTAC GT^[AC][GT]AC
Alw44I 1107 GTGCAC G^TGCAC
Aor51HI 1058 AGCGCT AGC^GCT
ApaLI 1107 GTGCAC G^TGCAC
AspI 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 929 TGCGCA TGC^GCA
BalI 89 TGGCCA TGG^CCA
BcgI 1109 GCACGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 461 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI 400 GAGAC GTCTC(1/5)
BsmBI 400 GAGACG CGTCTC(1/5)
BsmFI 524 GGGAC GGGAC(10/14)
BsmI 307 GAATGC GAATGC(1/-1)
Bsp1407I 689 TGTACA T^GTACA
BspHI 650 TCATGA T^CATGA
BsrBI 601 GAGCGG CCGCTC(-3/-3)
BsrGI 689 TGTACA T^GTACA
BssHII 502 GCGCGC G^CGCGC
BstAPI 1100 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 1116 GATC ^GATC
DraII 512 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 1058 AGCGCT AGC^GCT
EcoO109I 512 AGGACCT [GA]G^G[ACGT]CC[CT]
FseI 672 GGCCGGCC GGCCGG^CC
FspI 929 TGCGCA TGC^GCA
HaeII 1058 AGCGCT [GA]GCGC^[CT]
HgaI 592 GACGC GACGC(5/10)
HincII 859 GTCAAC GT[CT]^[GA]AC
HindII 859 GTCAAC GT[CT]^[GA]AC
KspI 520 CCGCGG CCGC^GG
MboI 1116 GATC ^GATC
MluNI 89 TGGCCA TGG^CCA
MscI 89 TGGCCA TGG^CCA
MseI 22 TTAA T^TAA
MslI 645 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 673 GCCGGC GCC^GGC
NcoI 720 CCATGG C^CATGG
NdeII 1116 GATC ^GATC
NgoMIV 673 GCCGGC G^CCGGC
NlaIV 625 GGCTCC GG[ACGT]^[ACGT]CC
PflFI 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 128 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 512 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 763 CAGCTG CAG^CTG
RcaI 650 TCATGA T^CATGA
SacI 1006 GAGCTC GAGCT^C
SacII 520 CCGCGG CCGC^GG
Sau3AI 1116 GATC ^GATC
SexAI 893 ACCTGGT A^CC[AT]GGT
SmlI 227 CTCAAG C^T[CT][GA]AG
SspBI 689 TGTACA T^GTACA
SspI 18 AATATT AAT^ATT
Tru9I 22 TTAA T^TAA
Tth111I 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 128 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 592 GACGCC G[GA]^CG[CT]C
981 GACGTC
BanII 624 GGGCTC G[GA]GC[CT]^C
1006 GAGCTC
BcnI 453 CCGGG CC^[GC]GG
840 CCGGG
BfaI 79 CTAG C^TAG
145 CTAG
BsaHI 592 GACGCC G[GA]^CG[CT]C
981 GACGTC
BsgI 658 CTGCAC GTGCAG(16/14)
1048 GTGCAG
BsiEI 675 CGGCCG CG[GA][CT]^CG
1020 CGGCCG
BsrDI 58 GCAATG GCAATG(2/0)
932 GCAATG
BsrFI 673 GCCGGC [GA]^CCGG[CT]
943 ACCGGC
Cfr10I 673 GCCGGC [GA]^CCGG[CT]
943 ACCGGC
EagI 675 CGGCCG C^GGCCG
1020 CGGCCG
EclXI 675 CGGCCG C^GGCCG
1020 CGGCCG
Eco52I 675 CGGCCG C^GGCCG
1020 CGGCCG
Hin1I 592 GACGCC G[GA]^CG[CT]C
981 GACGTC
MaeI 79 CTAG C^TAG
145 CTAG
MaeII 982 ACGT A^CGT
1091 ACGT
MspA1I 520 CCGCGG C[AC]G^C[GT]G
763 CAGCTG
NciI 453 CCGGG CC^[GC]GG
840 CCGGG
NspI 266 ACATGC [GA]CATG^[CT]
751 ACATGC
XcmI 578 CCAAGGAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
884 CCACACAAGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboII MnlI
MspI MvaI MvnI MwoI NlaIII
PstI RsaI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp509I
TspRI TthHB8I