Restriction map for
pF1KE2567
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BamHI
BciVI BclI BfrI BlnI BsaWI
BseAI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 771 GGTACC G^GTACC
AccI 2224 GTCTAC GT^[AC][GT]AC
AcsI 91 GAATTC [GA]^AATT[CT]
AflIII 1265 ACGTGT A^C[GA][CT]GT
AhdI 173 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1965 GATCC GGATC(4/5)
AlwNI 379 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1014 CCTGAGG CC^T[ACGT]AGG
ApaI 946 GGGCCC GGGCC^C
ApoI 91 GAATTC [GA]^AATT[CT]
Asp718I 771 GGTACC G^GTACC
AspEI 173 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 146 TGGCCA TGG^CCA
BbrPI 1264 CACGTG CAC^GTG
BbvCI 1634 CCTCAGC CCTCAGC(-5/-2)
BcgI 574 GCATCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 817 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 68 AGATCT A^GATCT
BlpI 490 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 490 GCTCAGC GC^T[ACGT]AGC
BsaAI 1264 CACGTG [CT]AC^GT[GA]
BsaBI 700 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1088 GAGACC GGTCTC(1/5)
BsmBI 1212 CGTCTC CGTCTC(1/5)
BsmI 782 GCATTC GAATGC(1/-1)
Bsp1407I 1415 TGTACA T^GTACA
BsrGI 1415 TGTACA T^GTACA
BstAPI 300 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1014 CCTGAGG CC^T[ACGT]AGG
CelII 490 GCTCAGC GC^T[ACGT]AGC
Eam1105I 173 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1215 CTCTTC CTCTTC(1/4)
Eco81I 1014 CCTGAGG CC^T[ACGT]AGG
EcoRI 91 GAATTC G^AATTC
EcoT22I 220 ATGCAT ATGCA^T
KpnI 771 GGTACC GGTAC^C
Ksp632I 1215 CTCTTC CTCTTC(1/4)
KspI 1333 CCGCGG CCGC^GG
MamI 700 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 146 TGGCCA TGG^CCA
MscI 146 TGGCCA TGG^CCA
MslI 213 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1248 GCCGGC GCC^GGC
NcoI 1365 CCATGG C^CATGG
NgoMIV 1248 GCCGGC G^CCGGC
NsiI 220 ATGCAT ATGCA^T
NspI 1410 GCATGT [GA]CATG^[CT]
PmaCI 1264 CACGTG CAC^GTG
PmlI 1264 CACGTG CAC^GTG
PshAI 1737 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 862 GAGCTC GAGCT^C
SacII 1333 CCGCGG CCGC^GG
SexAI 800 ACCTGGT A^CC[AT]GGT
SmaI 943 CCCGGG CCC^GGG
SmlI 124 CTCAAG C^T[CT][GA]AG
SspBI 1415 TGTACA T^GTACA
TfiI 1731 GAATC G^A[AT]TC
Tsp509I 92 AATT ^AATT
XmaCI 943 CCCGGG C^CCGGG
XmaI 943 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 1003 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1029 GACTCTGTC
BbeI 1599 GGCGCC GGCGC^C
1771 GGCGCC
BbsI 156 GAAGAC GAAGAC(2/6)
688 GAAGAC
BpuAI 156 GAAGAC GAAGAC(2/6)
688 GAAGAC
BsiWI 200 CGTACG C^GTACG
2102 CGTACG
BsrBI 28 CCGCTC CCGCTC(-3/-3)
252 GAGCGG
BsrFI 1248 GCCGGC [GA]^CCGG[CT]
1290 GCCGGT
BssSI 2008 CACGAG CACGAG(-5/-1)
598 CTCGTG
BstEII 336 GGTCACC G^GT[ACGT]ACC
1386 GGTCACC
BstPI 336 GGTCACC G^GT[ACGT]ACC
1386 GGTCACC
BstYI 68 AGATCT [GA]^GATC[CT]
1964 AGATCC
Cfr10I 1248 GCCGGC [GA]^CCGG[CT]
1290 GCCGGT
EcoNI 282 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1851 CCTGCGGGAGG
EcoO65I 336 GGTCACC G^GT[ACGT]ACC
1386 GGTCACC
HaeII 1599 GGCGCC [GA]GCGC^[CT]
1771 GGCGCC
KasI 1599 GGCGCC G^GCGCC
1771 GGCGCC
MflI 68 AGATCT [GA]^GATC[CT]
1964 AGATCC
NarI 1599 GGCGCC GG^CGCC
1771 GGCGCC
PflFI 1003 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1029 GACTCTGTC
SfoI 1599 GGCGCC GGC^GCC
1771 GGCGCC
TaqI 58 TCGA T^CGA
1490 TCGA
Tth111I 1003 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1029 GACTCTGTC
TthHB8I 58 TCGA T^CGA
1490 TCGA
XcmI 1469 CCAAGACGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1583 CCATTTGGGACCTGG
XhoII 68 AGATCT [GA]^GATC[CT]
1964 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI