Restriction map for
pF1KA1305
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbeI BciVI BfrI BglII BlnI
BsiWI BsmBI Bsp1407I BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI FspI HpaI KasI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 5359 GACGTC GACGT^C
Acc65I 2309 GGTACC G^GTACC
AccII 3861 CGCG CG^CG
AflIII 3932 ACATGT A^C[GA][CT]GT
Alw44I 1735 GTGCAC G^TGCAC
Aor51HI 4088 AGCGCT AGC^GCT
ApaLI 1735 GTGCAC G^TGCAC
Asp718I 2309 GGTACC G^GTACC
BamHI 503 GGATCC G^GATCC
BbrPI 3502 CACGTG CAC^GTG
BclI 809 TGATCA T^GATCA
BsaAI 3502 CACGTG [CT]AC^GT[GA]
BsaBI 2697 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4829 CGACCG CG[GA][CT]^CG
BspDI 2389 ATCGAT AT^CGAT
BspHI 383 TCATGA T^CATGA
BspLU11I 3932 ACATGT A^CATGT
BsrBI 1535 CCGCTC CCGCTC(-3/-3)
BsrDI 4473 CATTGC GCAATG(2/0)
BsrFI 5561 ACCGGC [GA]^CCGG[CT]
BssSI 2314 CACGAG CACGAG(-5/-1)
BstUI 3861 CGCG CG^CG
Cfr10I 5561 ACCGGC [GA]^CCGG[CT]
ClaI 2389 ATCGAT AT^CGAT
DraIII 4926 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1587 GACAGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4088 AGCGCT AGC^GCT
EcoRI 5161 GAATTC G^AATTC
FbaI 809 TGATCA T^GATCA
HaeII 4088 AGCGCT [GA]GCGC^[CT]
HincII 4827 GTCGAC GT[CT]^[GA]AC
HindII 4827 GTCGAC GT[CT]^[GA]AC
HindIII 3755 AAGCTT A^AGCTT
KpnI 2309 GGTACC GGTAC^C
KspI 3860 CCGCGG CCGC^GG
MamI 2697 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3861 CGCG CG^CG
PaeR7I 163 CTCGAG C^TCGAG
PmaCI 3502 CACGTG CAC^GTG
PmlI 3502 CACGTG CAC^GTG
RcaI 383 TCATGA T^CATGA
SacII 3860 CCGCGG CCGC^GG
SalI 4827 GTCGAC G^TCGAC
SbfI 1909 CCTGCAGG CCTGCA^GG
SphI 519 GCATGC GCATG^C
Sse8387I 1909 CCTGCAGG CCTGCA^GG
XhoI 163 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1295 GTAGAC GT^[AC][GT]AC
4827 GTCGAC
AccIII 651 TCCGGA T^CCGGA
4619 TCCGGA
AcsI 768 AAATTC [GA]^AATT[CT]
5161 GAATTC
AcyI 3069 GGCGTC G[GA]^CG[CT]C
5359 GACGTC
AocI 3724 CCTGAGG CC^T[ACGT]AGG
4821 CCTCAGG
ApoI 768 AAATTC [GA]^AATT[CT]
5161 GAATTC
Asp700I 117 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2769 GAATCTCTTC
AspI 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5488 GACCAGGTC
BcgI 4467 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2259 GCAGCCACCTCG
BsaHI 3069 GGCGTC G[GA]^CG[CT]C
5359 GACGTC
BseAI 651 TCCGGA T^CCGGA
4619 TCCGGA
BsmI 1122 GCATTC GAATGC(1/-1)
4942 GCATTC
BspEI 651 TCCGGA T^CCGGA
4619 TCCGGA
Bsu36I 3724 CCTGAGG CC^T[ACGT]AGG
4821 CCTCAGG
Eco81I 3724 CCTGAGG CC^T[ACGT]AGG
4821 CCTCAGG
Hin1I 3069 GGCGTC G[GA]^CG[CT]C
5359 GACGTC
MroI 651 TCCGGA T^CCGGA
4619 TCCGGA
PflFI 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5488 GACCAGGTC
Tth111I 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5488 GACCAGGTC
XmnI 117 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2769 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfiI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI