Restriction map for
pF1KE2466
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BlnI BseAI BsiWI BsmBI
BsmI BspDI BspEI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoO65I EcoRV FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AcsI 1848 GAATTC [GA]^AATT[CT]
Aor51HI 371 AGCGCT AGC^GCT
ApoI 1848 GAATTC [GA]^AATT[CT]
AseI 550 ATTAAT AT^TAAT
AsnI 550 ATTAAT AT^TAAT
BclI 2195 TGATCA T^GATCA
BsaWI 227 ACCGGA [AT]^CCGG[AT]
BspLU11I 2456 ACATGT A^CATGT
BstAPI 2823 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1128 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 40 GACCGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 371 AGCGCT AGC^GCT
EcoRI 1848 GAATTC G^AATTC
EcoT22I 1980 ATGCAT ATGCA^T
FbaI 2195 TGATCA T^GATCA
NsiI 1980 ATGCAT ATGCA^T
NspI 2456 ACATGT [GA]CATG^[CT]
PflMI 2691 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 231 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 550 ATTAAT AT^TAAT
ScaI 17 AGTACT AGT^ACT
Van91I 2691 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AspI 952 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACTGGGTC
BalI 815 TGGCCA TGG^CCA
1643 TGGCCA
BfaI 1361 CTAG C^TAG
1717 CTAG
BglII 755 AGATCT A^GATCT
2579 AGATCT
BsaAI 1870 TACGTG [CT]AC^GT[GA]
1915 TACGTG
BsaBI 1020 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATCGGCATC
BsaI 556 GAGACC GGTCTC(1/5)
1560 GGTCTC
BsiEI 32 CGGCCG CG[GA][CT]^CG
171 CGGCCG
BspHI 1574 TCATGA T^CATGA
2708 TCATGA
BsrBI 427 GAGCGG CCGCTC(-3/-3)
2854 GAGCGG
EagI 32 CGGCCG C^GGCCG
171 CGGCCG
EclXI 32 CGGCCG C^GGCCG
171 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
171 CGGCCG
EcoNI 402 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1734 CCTGGACGAGG
HaeII 371 AGCGCT [GA]GCGC^[CT]
1427 AGCGCC
HindIII 1800 AAGCTT A^AGCTT
2383 AAGCTT
MaeI 1361 CTAG C^TAG
1717 CTAG
MamI 1020 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATCGGCATC
MluNI 815 TGGCCA TGG^CCA
1643 TGGCCA
MscI 815 TGGCCA TGG^CCA
1643 TGGCCA
MseI 551 TTAA T^TAA
1475 TTAA
NaeI 173 GCCGGC GCC^GGC
1430 GCCGGC
NgoMIV 173 GCCGGC G^CCGGC
1430 GCCGGC
PflFI 952 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACTGGGTC
PpuMI 1313 GGGACCT [GA]G^G[AT]CC[CT]
1781 AGGTCCC
PstI 1155 CTGCAG CTGCA^G
2583 CTGCAG
PvuII 2014 CAGCTG CAG^CTG
2827 CAGCTG
RcaI 1574 TCATGA T^CATGA
2708 TCATGA
SacI 1173 GAGCTC GAGCT^C
2311 GAGCTC
SexAI 953 ACCTGGT A^CC[AT]GGT
2696 ACCTGGT
SmaI 674 CCCGGG CCC^GGG
1905 CCCGGG
Tru9I 551 TTAA T^TAA
1475 TTAA
Tsp509I 1499 AATT ^AATT
1849 AATT
Tth111I 952 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACTGGGTC
XmaCI 674 CCCGGG C^CCGGG
1905 CCCGGG
XmaI 674 CCCGGG C^CCGGG
1905 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII