Restriction map for
pF1KE2657
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BseRI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MscI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 520 TCCGGA T^CCGGA
AcyI 222 GGCGCC G[GA]^CG[CT]C
Alw44I 91 GTGCAC G^TGCAC
ApaLI 91 GTGCAC G^TGCAC
AvaI 59 CCCGGG C^[CT]CG[GA]G
BbeI 222 GGCGCC GGCGC^C
BglI 65 GCCGATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 509 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 509 GCTCAGC GC^T[ACGT]AGC
BsaHI 222 GGCGCC G[GA]^CG[CT]C
BsaI 169 GGTCTC GGTCTC(1/5)
BsaWI 520 TCCGGA [AT]^CCGG[AT]
BseAI 520 TCCGGA T^CCGGA
BsmAI 170 GTCTC GTCTC(1/5)
BsmFI 527 GGGAC GGGAC(10/14)
BsoBI 59 CCCGGG C^[CT]CG[GA]G
BspEI 520 TCCGGA T^CCGGA
BspHI 368 TCATGA T^CATGA
BspMI 355 ACCTGC ACCTGC(4/8)
BsrBI 577 CCGCTC CCGCTC(-3/-3)
BssSI 301 CTCGTG CACGAG(-5/-1)
BstEII 264 GGTTACC G^GT[ACGT]ACC
BstPI 264 GGTTACC G^GT[ACGT]ACC
CelII 509 GCTCAGC GC^T[ACGT]AGC
CfoI 223 GCGC GCG^C
DpnII 330 GATC ^GATC
DraIII 558 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 548 TGGCCG [CT]^GGCC[GA]
EcoO65I 264 GGTTACC G^GT[ACGT]ACC
HaeII 222 GGCGCC [GA]GCGC^[CT]
HhaI 223 GCGC GCG^C
Hin1I 222 GGCGCC G[GA]^CG[CT]C
HinP1I 223 GCGC G^CGC
HphI 144 GGTGA GGTGA(8/7)
KasI 222 GGCGCC G^GCGCC
MaeII 472 ACGT A^CGT
MaeIII 265 GTTAC ^GT[ACGT]AC
MboI 330 GATC ^GATC
MboII 372 GAAGA GAAGA(8/7)
MroI 520 TCCGGA T^CCGGA
MspA1I 322 CAGCTG C[AC]G^C[GT]G
NarI 222 GGCGCC GG^CGCC
NdeII 330 GATC ^GATC
PflMI 343 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 448 CTGCAG CTGCA^G
PvuII 322 CAGCTG CAG^CTG
RcaI 368 TCATGA T^CATGA
Sau3AI 330 GATC ^GATC
SexAI 260 ACCTGGT A^CC[AT]GGT
SfcI 448 CTGCAG C^T[GA][CT]AG
SfoI 222 GGCGCC GGC^GCC
SmaI 59 CCCGGG CCC^GGG
SmlI 400 CTTGAG C^T[CT][GA]AG
Van91I 343 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 293 CCACTGGCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AspHI 91 GTGCAC G[AT]GC[AT]^C
256 GAGCAC
BanII 33 GAGCCC G[GA]GC[CT]^C
155 GAGCCC
BpmI 380 CTCCAG CTGGAG(16/14)
205 CTGGAG
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
256 GAGCAC
BsmI 439 GAATGC GAATGC(1/-1)
74 GCATTC
BstAPI 74 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
353 GCACCTGCTGC
BstF5I 390 CATCC GGATG(2/0)
3 GGATG
DraII 20 AGGTCCT [GA]G^G[ACGT]CC[CT]
527 GGGACCC
EcoO109I 20 AGGTCCT [GA]G^G[ACGT]CC[CT]
527 GGGACCC
FauI 36 CCCGC CCCGC(4/6)
460 CCCGC
FokI 390 CATCC GGATG(9/13)
3 GGATG
PpuMI 20 AGGTCCT [GA]G^G[AT]CC[CT]
527 GGGACCC
SfaNI 502 GATGC GCATC(5/9)
389 GCATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HpaII ItaI MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TseI
TspRI