Restriction map for
pF1KE2454
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BfrI BglII BlnI BpmI BsaBI
BsaHI BsiWI BspDI BspMI BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MscI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 134 TCCGGA T^CCGGA
AflIII 1454 ACATGT A^C[GA][CT]GT
AlwNI 1751 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 478 CCTAAGG CC^T[ACGT]AGG
ApaI 524 GGGCCC GGGCC^C
AspI 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 341 CCTCAGC CCTCAGC(-5/-2)
BciVI 924 GGATAC GTATCC(6/5)
BclI 1797 TGATCA T^GATCA
BlpI 409 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 409 GCTCAGC GC^T[ACGT]AGC
BsaAI 1195 TACGTG [CT]AC^GT[GA]
BsaI 514 GGTCTC GGTCTC(1/5)
BsaWI 134 TCCGGA [AT]^CCGG[AT]
BseAI 134 TCCGGA T^CCGGA
BsgI 403 CTGCAC GTGCAG(16/14)
BsiEI 329 CGGCCG CG[GA][CT]^CG
BsmBI 57 CGTCTC CGTCTC(1/5)
Bsp1407I 1483 TGTACA T^GTACA
BspEI 134 TCCGGA T^CCGGA
BspHI 2115 TCATGA T^CATGA
BspLU11I 1454 ACATGT A^CATGT
BsrGI 1483 TGTACA T^GTACA
BssSI 992 CACGAG CACGAG(-5/-1)
BstXI 1059 CCAGCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 478 CCTAAGG CC^T[ACGT]AGG
CelII 409 GCTCAGC GC^T[ACGT]AGC
EaeI 329 CGGCCG [CT]^GGCC[GA]
EagI 329 CGGCCG C^GGCCG
EclXI 329 CGGCCG C^GGCCG
Eco52I 329 CGGCCG C^GGCCG
Eco81I 478 CCTAAGG CC^T[ACGT]AGG
EcoNI 1063 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1797 TGATCA T^GATCA
HindIII 1398 AAGCTT A^AGCTT
MroI 134 TCCGGA T^CCGGA
MslI 1487 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1492 CCATGG C^CATGG
NspI 1454 ACATGT [GA]CATG^[CT]
PflFI 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1550 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 101 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 2115 TCATGA T^CATGA
SacI 1706 GAGCTC GAGCT^C
SspBI 1483 TGTACA T^GTACA
SspI 2308 AATATT AAT^ATT
Tth111I 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1550 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 22 GTGCAC G^TGCAC
1777 GTGCAC
AlwI 1581 GATCC GGATC(4/5)
2370 GGATC
ApaLI 22 GTGCAC G^TGCAC
1777 GTGCAC
BanI 153 GGCACC G^G[CT][GA]CC
369 GGTGCC
BbsI 733 GAAGAC GAAGAC(2/6)
1693 GAAGAC
BcgI 14 CGAGCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
217 GCAGCACCTTCG
BglI 526 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
535 GCCGGCGGGGC
BpuAI 733 GAAGAC GAAGAC(2/6)
1693 GAAGAC
BsmI 1865 GAATGC GAATGC(1/-1)
1952 GCATTC
BsrI 1276 CCAGT ACTGG(1/-1)
1899 CCAGT
BstF5I 882 CATCC GGATG(2/0)
2343 GGATG
CfoI 375 GCGC GCG^C
471 GCGC
FokI 882 CATCC GGATG(9/13)
2343 GGATG
HhaI 375 GCGC GCG^C
471 GCGC
HinP1I 375 GCGC G^CGC
471 GCGC
KspI 38 CCGCGG CCGC^GG
157 CCGCGG
NaeI 213 GCCGGC GCC^GGC
535 GCCGGC
NgoMIV 213 GCCGGC G^CCGGC
535 GCCGGC
PleI 1465 GAGTC GAGTC(4/5)
1524 GAGTC
PstI 205 CTGCAG CTGCA^G
1184 CTGCAG
SacII 38 CCGCGG CCGC^GG
157 CCGCGG
SfiI 525 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
534 GGCCGGCGGGGCC
SmaI 98 CCCGGG CCC^GGG
548 CCCGGG
TaqI 1527 TCGA T^CGA
1662 TCGA
Tsp45I 1467 GTCAC ^GT[GC]AC
1605 GTCAC
TthHB8I 1527 TCGA T^CGA
1662 TCGA
XmaCI 98 CCCGGG C^CCGGG
548 CCCGGG
XmaI 98 CCCGGG C^CCGGG
548 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstNI BstUI Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI XmnI