Restriction map for
pF1KE2446
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BbeI
BbvCI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AlwNI 772 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 999 CCTTAGG CC^T[ACGT]AGG
ApaI 1178 GGGCCC GGGCC^C
AspI 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 52 CCCGAG C^[CT]CG[GA]G
AviII 599 TGCGCA TGC^GCA
BalI 849 TGGCCA TGG^CCA
BamHI 537 GGATCC G^GATCC
BbrPI 1650 CACGTG CAC^GTG
BbsI 1899 GAAGAC GAAGAC(2/6)
BcgI 1398 GCAGCTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1824 GGATAC GTATCC(6/5)
BglI 1681 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1899 GAAGAC GAAGAC(2/6)
BsaAI 1650 CACGTG [CT]AC^GT[GA]
BsaWI 1088 ACCGGA [AT]^CCGG[AT]
BsgI 12 CTGCAC GTGCAG(16/14)
BsoBI 52 CCCGAG C^[CT]CG[GA]G
BspHI 695 TCATGA T^CATGA
BstEII 1884 GGTAACC G^GT[ACGT]ACC
BstPI 1884 GGTAACC G^GT[ACGT]ACC
Bsu36I 999 CCTTAGG CC^T[ACGT]AGG
DraI 582 TTTAAA TTT^AAA
DrdI 1087 GACCGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 999 CCTTAGG CC^T[ACGT]AGG
EcoNI 971 CCTCATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1884 GGTAACC G^GT[ACGT]ACC
EcoRI 613 GAATTC G^AATTC
EcoT14I 1568 CCTTGG C^C[AT][AT]GG
FauI 93 GCGGG CCCGC(4/6)
FspI 599 TGCGCA TGC^GCA
HaeII 48 AGCGCC [GA]GCGC^[CT]
HincII 715 GTCAAC GT[CT]^[GA]AC
HindII 715 GTCAAC GT[CT]^[GA]AC
HindIII 586 AAGCTT A^AGCTT
HphI 1158 TCACC GGTGA(8/7)
MluNI 849 TGGCCA TGG^CCA
MscI 849 TGGCCA TGG^CCA
NheI 679 GCTAGC G^CTAGC
NspI 1364 ACATGC [GA]CATG^[CT]
PflFI 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 660 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1650 CACGTG CAC^GTG
PmlI 1650 CACGTG CAC^GTG
PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1633 CTGCAG CTGCA^G
RcaI 695 TCATGA T^CATGA
SacI 1672 GAGCTC GAGCT^C
ScaI 965 AGTACT AGT^ACT
SmlI 1411 CTTGAG C^T[CT][GA]AG
StyI 1568 CCTTGG C^C[AT][AT]GG
Tth111I 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 660 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1757 CCAGAGACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 746 GTAC GT^AC
966 GTAC
AflIII 1649 ACACGT A^C[GA][CT]GT
1651 ACGTGT
AspHI 173 GTGCTC G[AT]GC[AT]^C
1672 GAGCTC
BanI 34 GGTGCC G^G[CT][GA]CC
2114 GGCACC
BanII 1178 GGGCCC G[GA]GC[CT]^C
1672 GAGCTC
BcnI 84 CCCGG CC^[GC]GG
1071 CCGGG
BglII 824 AGATCT A^GATCT
1743 AGATCT
BsaJI 53 CCGAGG C^C[ACGT][ACGT]GG
1568 CCTTGG
BsiHKAI 173 GTGCTC G[AT]GC[AT]^C
1672 GAGCTC
BsrDI 1443 CATTGC GCAATG(2/0)
1418 GCAATG
DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT]
1177 AGGGCCC
EaeI 849 TGGCCA [CT]^GGCC[GA]
1688 TGGCCG
EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT]
1177 AGGGCCC
NciI 84 CCCGG CC^[GC]GG
1071 CCGGG
RsaI 746 GTAC GT^AC
966 GTAC
SapI 902 GCTCTTC GCTCTTC(1/4)
2005 GCTCTTC
SfcI 788 CTGTAG C^T[GA][CT]AG
1633 CTGCAG
TfiI 331 GAATC G^A[AT]TC
1053 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BbvI BfaI BmyI
BpmI BsaI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII