Restriction map for
pF1KE2758
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MslI MspA1I MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 755 GAATTC [GA]^AATT[CT]
ApoI 755 GAATTC [GA]^AATT[CT]
AvaI 787 CCCGGG C^[CT]CG[GA]G
AvrII 487 CCTAGG C^CTAGG
BlnI 487 CCTAGG C^CTAGG
BseRI 401 GAGGAG GAGGAG(10/8)
BsmBI 863 GAGACG CGTCTC(1/5)
BsoBI 787 CCCGGG C^[CT]CG[GA]G
BspMI 1690 GCAGGT ACCTGC(4/8)
BsrBI 191 CCGCTC CCGCTC(-3/-3)
DraIII 1805 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 131 TGGCCG [CT]^GGCC[GA]
EcoRI 755 GAATTC G^AATTC
HgaI 865 GACGC GACGC(5/10)
MfeI 1918 CAATTG C^AATTG
MunI 1918 CAATTG C^AATTG
NdeI 310 CATATG CA^TATG
SacI 1226 GAGCTC GAGCT^C
SbfI 1687 CCTGCAGG CCTGCA^GG
SfaNI 1906 GATGC GCATC(5/9)
SmaI 787 CCCGGG CCC^GGG
Sse8387I 1687 CCTGCAGG CCTGCA^GG
TaqI 197 TCGA T^CGA
TthHB8I 197 TCGA T^CGA
XcmI 1608 CCATGGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 787 CCCGGG C^CCGGG
XmaI 787 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 433 CCTCAGG CC^T[ACGT]AGG
829 CCTAAGG
AspHI 746 GAGCAC G[AT]GC[AT]^C
1226 GAGCTC
BclI 477 TGATCA T^GATCA
531 TGATCA
BsiHKAI 746 GAGCAC G[AT]GC[AT]^C
1226 GAGCTC
BsmAI 863 GAGAC GTCTC(1/5)
1454 GAGAC
BsmI 656 GAATGC GAATGC(1/-1)
1553 GAATGC
BstEII 565 GGTGACC G^GT[ACGT]ACC
907 GGTGACC
BstPI 565 GGTGACC G^GT[ACGT]ACC
907 GGTGACC
BstYI 46 AGATCC [GA]^GATC[CT]
1399 GGATCT
Bsu36I 433 CCTCAGG CC^T[ACGT]AGG
829 CCTAAGG
DrdI 422 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1235 GACCAAGGGGTC
EarI 398 GAAGAG CTCTTC(1/4)
1112 GAAGAG
Eco81I 433 CCTCAGG CC^T[ACGT]AGG
829 CCTAAGG
EcoO65I 565 GGTGACC G^GT[ACGT]ACC
907 GGTGACC
FbaI 477 TGATCA T^GATCA
531 TGATCA
Ksp632I 398 GAAGAG CTCTTC(1/4)
1112 GAAGAG
MflI 46 AGATCC [GA]^GATC[CT]
1399 GGATCT
PleI 1219 GACTC GAGTC(4/5)
1436 GACTC
PstI 926 CTGCAG CTGCA^G
1688 CTGCAG
SfcI 926 CTGCAG C^T[GA][CT]AG
1688 CTGCAG
TspRI 125 CAGTG CA[GC]TG[ACGT][ACGT]^
1275 CACTG
XhoII 46 AGATCC [GA]^GATC[CT]
1399 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SexAI SfiI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I