Restriction map for
pF1KB4448
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BbvI
BcgI BciVI BfaI BfrI BglII
BlnI BpmI BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
Fnu4HI FseI FspI HincII HindII
HindIII HpaI ItaI KasI KspI
MaeI MaeIII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 292 GGTACC G^GTACC
Asp718I 292 GGTACC G^GTACC
AvaI 237 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BclI 491 TGATCA T^GATCA
BglI 37 GCCCTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 487 TACGTG [CT]AC^GT[GA]
BsaI 307 GAGACC GGTCTC(1/5)
BsmAI 307 GAGAC GTCTC(1/5)
BsmFI 390 GTCCC GGGAC(10/14)
BsmI 112 GCATTC GAATGC(1/-1)
BsoBI 237 CTCGGG C^[CT]CG[GA]G
BspDI 448 ATCGAT AT^CGAT
BsrBI 288 GAGCGG CCGCTC(-3/-3)
BsrI 435 CCAGT ACTGG(1/-1)
BstF5I 59 CATCC GGATG(2/0)
BstXI 446 CCATCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 92 AGATCC [GA]^GATC[CT]
CfoI 478 GCGC GCG^C
ClaI 448 ATCGAT AT^CGAT
DsaI 498 CCACGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 415 GAAGAG CTCTTC(1/4)
FauI 457 GCGGG CCCGC(4/6)
FbaI 491 TGATCA T^GATCA
FokI 59 CATCC GGATG(9/13)
HaeII 477 GGCGCT [GA]GCGC^[CT]
HhaI 478 GCGC GCG^C
HinP1I 478 GCGC G^CGC
HinfI 87 GACTC G^A[ACGT]TC
KpnI 292 GGTACC GGTAC^C
Ksp632I 415 GAAGAG CTCTTC(1/4)
MflI 92 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 317 TTAA T^TAA
PleI 87 GACTC GAGTC(4/5)
PpuMI 158 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 192 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 330 GATGC GCATC(5/9)
StuI 233 AGGCCT AGG^CCT
TaqI 449 TCGA T^CGA
Tru9I 317 TTAA T^TAA
TthHB8I 449 TCGA T^CGA
XhoII 92 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 217 GACGCC G[GA]^CG[CT]C
298 GACGCC
AfaI 293 GTAC GT^AC
369 GTAC
AlwI 93 GATCC GGATC(4/5)
126 GGATC
ApaI 44 GGGCCC GGGCC^C
241 GGGCCC
BanI 34 GGTGCC G^G[CT][GA]CC
292 GGTACC
BanII 44 GGGCCC G[GA]GC[CT]^C
241 GGGCCC
BbsI 12 GAAGAC GAAGAC(2/6)
67 GTCTTC
BcnI 42 CCGGG CC^[GC]GG
339 CCCGG
BlpI 285 GCTGAGC GC^T[ACGT]AGC
480 GCTCAGC
Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC
480 GCTCAGC
BpuAI 12 GAAGAC GAAGAC(2/6)
67 GTCTTC
BsaHI 217 GACGCC G[GA]^CG[CT]C
298 GACGCC
BsiYI 270 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCACGGGGAGG
BslI 270 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCACGGGGAGG
Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC
457 GCGGGC
CelII 285 GCTGAGC GC^T[ACGT]AGC
480 GCTCAGC
DraII 158 AGGACCT [GA]G^G[ACGT]CC[CT]
240 GGGGCCC
EcoO109I 158 AGGACCT [GA]G^G[ACGT]CC[CT]
240 GGGGCCC
HapII 42 CCGG C^CGG
340 CCGG
Hin1I 217 GACGCC G[GA]^CG[CT]C
298 GACGCC
HpaII 42 CCGG C^CGG
340 CCGG
HphI 254 TCACC GGTGA(8/7)
494 TCACC
MaeII 197 ACGT A^CGT
488 ACGT
MslI 117 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CATCGATGTG
MspI 42 CCGG C^CGG
340 CCGG
MwoI 37 GCCCTCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
46 GCCCAGAGAGC
NciI 42 CCGGG CC^[GC]GG
339 CCCGG
RsaI 293 GTAC GT^AC
369 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI AvaII BmyI BsaJI
BseRI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EcoRII HaeIII HgaI
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI