Restriction map for
pF1KE2633
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 2053 CGCG CG^CG
AfaI 1564 GTAC GT^AC
AflIII 1187 ACATGT A^C[GA][CT]GT
Alw44I 89 GTGCAC G^TGCAC
AocI 399 CCTTAGG CC^T[ACGT]AGG
ApaI 12 GGGCCC GGGCC^C
ApaLI 89 GTGCAC G^TGCAC
Asp700I 367 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1455 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1239 CTCGGG C^[CT]CG[GA]G
BamHI 2501 GGATCC G^GATCC
BsaI 1661 GAGACC GGTCTC(1/5)
BsoBI 1239 CTCGGG C^[CT]CG[GA]G
BspLU11I 1187 ACATGT A^CATGT
BsrBI 2497 GAGCGG CCGCTC(-3/-3)
BsrDI 1247 CATTGC GCAATG(2/0)
BstBI 221 TTCGAA TT^CGAA
BstUI 2053 CGCG CG^CG
Bsu36I 399 CCTTAGG CC^T[ACGT]AGG
CfoI 4 GCGC GCG^C
CpoI 553 CGGTCCG CG^G[AT]CCG
DsaI 1113 CCGTGG C^C[GA][CT]GG
Eco81I 399 CCTTAGG CC^T[ACGT]AGG
EcoRI 1634 GAATTC G^AATTC
FauI 2031 GCGGG CCCGC(4/6)
HgaI 1459 GCGTC GACGC(5/10)
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HincII 1401 GTTAAC GT[CT]^[GA]AC
HindII 1401 GTTAAC GT[CT]^[GA]AC
HpaI 1401 GTTAAC GTT^AAC
MslI 2064 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2053 CGCG CG^CG
NspV 221 TTCGAA TT^CGAA
PflFI 1455 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 1564 GTAC GT^AC
RsrII 553 CGGTCCG CG^G[AT]CCG
SexAI 1664 ACCAGGT A^CC[AT]GGT
SfuI 221 TTCGAA TT^CGAA
SspI 259 AATATT AAT^ATT
Tth111I 1455 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 699 TCTAGA T^CTAGA
XcmI 1380 CCAGATGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 367 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 1218 GTCTAC GT^[AC][GT]AC
1589 GTAGAC
AseI 70 ATTAAT AT^TAAT
2065 ATTAAT
AsnI 70 ATTAAT AT^TAAT
2065 ATTAAT
AvaII 554 GGTCC G^G[AT]CC
1318 GGACC
BglII 1784 AGATCT A^GATCT
1820 AGATCT
BstAPI 1282 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1965 GCACAGCCTGC
DraI 1192 TTTAAA TTT^AAA
2154 TTTAAA
EcoT14I 1368 CCTTGG C^C[AT][AT]GG
2519 CCTTGG
HindIII 1259 AAGCTT A^AGCTT
2467 AAGCTT
MspA1I 229 CAGCTG C[AC]G^C[GT]G
1681 CAGCTG
PshBI 70 ATTAAT AT^TAAT
2065 ATTAAT
PvuII 229 CAGCTG CAG^CTG
1681 CAGCTG
SapI 1918 GAAGAGC GCTCTTC(1/4)
2338 GAAGAGC
StuI 638 AGGCCT AGG^CCT
1811 AGGCCT
StyI 1368 CCTTGG C^C[AT][AT]GG
2519 CCTTGG
TaqI 222 TCGA T^CGA
1774 TCGA
TthHB8I 222 TCGA T^CGA
1774 TCGA
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII