Restriction map for
pF1KE3491
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# NO CUT ENZYMES ( 94 enzymes) #
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Acc65I AccIII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BclI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiHKAI BsiWI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I FauI FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1483 GACGTC GACGT^C
AccII 2525 CGCG CG^CG
AciI 772 GCGG CCGC(-3/-1)
AclI 1592 AACGTT AA^CGTT
AcyI 1483 GACGTC G[GA]^CG[CT]C
AflII 2164 CTTAAG C^TTAAG
AocI 5509 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1847 AGCGCT AGC^GCT
BamHI 1309 GGATCC G^GATCC
BanI 4057 GGTGCC G^G[CT][GA]CC
BcgI 589 CGAAAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4647 GGATAC GTATCC(6/5)
BfrI 2164 CTTAAG C^TTAAG
BsaHI 1483 GACGTC G[GA]^CG[CT]C
BsaI 4618 GGTCTC GGTCTC(1/5)
BsaWI 4554 ACCGGA [AT]^CCGG[AT]
BsgI 1190 CTGCAC GTGCAG(16/14)
BsmBI 1481 GAGACG CGTCTC(1/5)
BspLU11I 546 ACATGT A^CATGT
BsrDI 1263 CATTGC GCAATG(2/0)
BssSI 3794 CACGAG CACGAG(-5/-1)
BstEII 202 GGTGACC G^GT[ACGT]ACC
BstPI 202 GGTGACC G^GT[ACGT]ACC
BstUI 2525 CGCG CG^CG
BstXI 3812 CCAAACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5509 CCTCAGG CC^T[ACGT]AGG
CfoI 1848 GCGC GCG^C
Eco47III 1847 AGCGCT AGC^GCT
Eco81I 5509 CCTCAGG CC^T[ACGT]AGG
EcoO65I 202 GGTGACC G^GT[ACGT]ACC
EcoRV 4909 GATATC GAT^ATC
HaeII 1847 AGCGCT [GA]GCGC^[CT]
HapII 4555 CCGG C^CGG
HgaI 3760 GCGTC GACGC(5/10)
HhaI 1848 GCGC GCG^C
Hin1I 1483 GACGTC G[GA]^CG[CT]C
HinP1I 1848 GCGC G^CGC
HpaII 4555 CCGG C^CGG
MspA1I 4667 CAGCTG C[AC]G^C[GT]G
MspI 4555 CCGG C^CGG
MvnI 2525 CGCG CG^CG
PflMI 4036 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1592 AACGTT AA^CGTT
PvuII 4667 CAGCTG CAG^CTG
StuI 4815 AGGCCT AGG^CCT
Van91I 4036 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1822 GTAGAC GT^[AC][GT]AC
3235 GTCTAC
AflIII 546 ACATGT A^C[GA][CT]GT
2576 ACACGT
AlwNI 1297 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4037 CAGAGGCTG
BanII 258 GAGCCC G[GA]GC[CT]^C
1027 GAGCCC
BbsI 4516 GAAGAC GAAGAC(2/6)
4797 GTCTTC
BmyI 258 GAGCCC G[AGT]GC[ACT]^C
1027 GAGCCC
BpuAI 4516 GAAGAC GAAGAC(2/6)
4797 GTCTTC
Bsp1286I 258 GAGCCC G[AGT]GC[ACT]^C
1027 GAGCCC
DraI 2443 TTTAAA TTT^AAA
4354 TTTAAA
DraII 71 AGGACCC [GA]G^G[ACGT]CC[CT]
2958 AGGACCT
EcoNI 1580 CCTATACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4726 CCTCCAAGAGG
EcoO109I 71 AGGACCC [GA]G^G[ACGT]CC[CT]
2958 AGGACCT
EcoRI 821 GAATTC G^AATTC
3587 GAATTC
MfeI 2555 CAATTG C^AATTG
2786 CAATTG
MunI 2555 CAATTG C^AATTG
2786 CAATTG
PpuMI 71 AGGACCC [GA]G^G[AT]CC[CT]
2958 AGGACCT
SapI 3520 GAAGAGC GCTCTTC(1/4)
4871 GAAGAGC
SexAI 2009 ACCTGGT A^CC[AT]GGT
4894 ACCTGGT
SphI 425 GCATGC GCATG^C
1391 GCATGC
XcmI 1409 CCATTCCGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4239 CCACTTGGAAACTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I AvaII BalI
BbvI BfaI BglII BpmI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MvaI
MwoI NcoI NdeI NdeII NheI
NlaIII NlaIV NsiI NspI PleI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI