Restriction map for
pF1KE1237
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BglII BlnI
BmyI BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
TseI TspRI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 481 GCGG CCGC(-3/-1)
AfaI 100 GTAC GT^AC
AflII 1081 CTTAAG C^TTAAG
BamHI 717 GGATCC G^GATCC
BfrI 1081 CTTAAG C^TTAAG
BlpI 261 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 261 GCTAAGC GC^T[ACGT]AGC
BsaBI 163 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 666 CCCAGG C^C[ACGT][ACGT]GG
BseRI 946 GAGGAG GAGGAG(10/8)
BspHI 519 TCATGA T^CATGA
BstXI 988 CCAGATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 717 GGATCC [GA]^GATC[CT]
Cac8I 1227 GCCAGC GC[ACGT]^[ACGT]GC
CelII 261 GCTAAGC GC^T[ACGT]AGC
EcoNI 1216 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 481 GCGGC GC^[ACGT]GC
HaeIII 1226 GGCC GG^CC
ItaI 481 GCGGC GC^[ACGT]GC
MaeII 1128 ACGT A^CGT
MamI 163 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 717 GGATCC [GA]^GATC[CT]
MspA1I 1229 CAGCTG C[AC]G^C[GT]G
MwoI 61 GCTCTTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PacI 349 TTAATTAA TTAAT^TAA
PflMI 142 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1229 CAGCTG CAG^CTG
RcaI 519 TCATGA T^CATGA
RsaI 100 GTAC GT^AC
SfcI 22 CTACAG C^T[GA][CT]AG
Van91I 142 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 717 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 46 ACATGT A^C[GA][CT]GT
897 ACATGT
AlwI 718 GATCC GGATC(4/5)
717 GGATC
AlwNI 143 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1153 CAGAAGCTG
AvaII 11 GGACC G^G[AT]CC
390 GGACC
BclI 417 TGATCA T^GATCA
1137 TGATCA
BsiYI 142 CCAGATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1216 CCTCCATCAGG
BslI 142 CCAGATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1216 CCTCCATCAGG
BsmAI 703 GAGAC GTCTC(1/5)
1000 GAGAC
BspLU11I 46 ACATGT A^CATGT
897 ACATGT
BssKI 667 CCAGG ^CC[ACGT]GG
780 CCTGG
BstNI 667 CCAGG CC^[AT]GG
780 CCTGG
DraI 529 TTTAAA TTT^AAA
573 TTTAAA
DraIII 112 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
289 CACCATGTG
EcoRI 976 GAATTC G^AATTC
1211 GAATTC
EcoRII 667 CCAGG ^CC[AT]GG
780 CCTGG
FbaI 417 TGATCA T^GATCA
1137 TGATCA
MaeIII 287 GTCAC ^GT[ACGT]AC
937 GTGAC
MvaI 667 CCAGG CC^[AT]GG
780 CCTGG
NlaIV 717 GGATCC GG[ACGT]^[ACGT]CC
984 GGAGCC
NspI 46 ACATGT [GA]CATG^[CT]
897 ACATGT
SapI 1117 GAAGAGC GCTCTTC(1/4)
400 GCTCTTC
ScrFI 667 CCAGG CC^[ACGT]GG
780 CCTGG
SmlI 172 CTCAAG C^T[CT][GA]AG
1081 CTTAAG
Tsp45I 287 GTCAC ^GT[GC]AC
937 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsrI
BstF5I Cfr13I DdeI DpnII EarI
FokI HinfI HphI Ksp632I MboI
MboII MnlI MseI NdeII NlaIII
PleI Sau3AI Sau96I SspI TfiI
Tru9I Tsp509I