Restriction map for
pF1KE1722
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 123 GTAC GT^AC
AhdI 370 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 322 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 370 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 41 TGGCCA TGG^CCA
BbeI 142 GGCGCC GGCGC^C
BglI 677 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 476 CTGGAG CTGGAG(16/14)
BsaI 535 GAGACC GGTCTC(1/5)
BseRI 565 GAGGAG GAGGAG(10/8)
BsmBI 377 CGTCTC CGTCTC(1/5)
BsmFI 260 GGGAC GGGAC(10/14)
BsmI 266 GCATTC GAATGC(1/-1)
BspMI 673 ACCTGC ACCTGC(4/8)
BssSI 442 CTCGTG CACGAG(-5/-1)
BstXI 146 CCAGTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 593 AGATCC [GA]^GATC[CT]
DraIII 526 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 370 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 424 CCCGC CCCGC(4/6)
HaeII 142 GGCGCC [GA]GCGC^[CT]
HgaI 353 GCGTC GACGC(5/10)
HincII 243 GTTGAC GT[CT]^[GA]AC
HindII 243 GTTGAC GT[CT]^[GA]AC
HinfI 226 GATTC G^A[ACGT]TC
HphI 420 TCACC GGTGA(8/7)
KasI 142 GGCGCC G^GCGCC
MflI 593 AGATCC [GA]^GATC[CT]
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
NarI 142 GGCGCC GG^CGCC
PpuMI 637 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 123 GTAC GT^AC
SfaNI 481 GATGC GCATC(5/9)
SfoI 142 GGCGCC GGC^GCC
StuI 569 AGGCCT AGG^CCT
TfiI 226 GATTC G^A[AT]TC
XhoII 593 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 142 GGCGCC G[GA]^CG[CT]C
352 GGCGTC
AlwI 384 GATCC GGATC(4/5)
594 GATCC
BbsI 651 GAAGAC GAAGAC(2/6)
355 GTCTTC
BcnI 78 CCGGG CC^[GC]GG
656 CCCGG
BpuAI 651 GAAGAC GAAGAC(2/6)
355 GTCTTC
BsaHI 142 GGCGCC G[GA]^CG[CT]C
352 GGCGTC
BsmAI 535 GAGAC GTCTC(1/5)
378 GTCTC
CfoI 143 GCGC GCG^C
166 GCGC
DdeI 83 CTCAG C^T[ACGT]AG
119 CTGAG
DpnII 384 GATC ^GATC
594 GATC
DsaI 614 CCATGG C^C[GA][CT]GG
629 CCATGG
EaeI 41 TGGCCA [CT]^GGCC[GA]
542 CGGCCA
EcoT14I 614 CCATGG C^C[AT][AT]GG
629 CCATGG
HapII 78 CCGG C^CGG
657 CCGG
HhaI 143 GCGC GCG^C
166 GCGC
Hin1I 142 GGCGCC G[GA]^CG[CT]C
352 GGCGTC
HinP1I 143 GCGC G^CGC
166 GCGC
HpaII 78 CCGG C^CGG
657 CCGG
MboI 384 GATC ^GATC
594 GATC
MboII 651 GAAGA GAAGA(8/7)
356 TCTTC
MspI 78 CCGG C^CGG
657 CCGG
NciI 78 CCGGG CC^[GC]GG
656 CCCGG
NcoI 614 CCATGG C^CATGG
629 CCATGG
NdeII 384 GATC ^GATC
594 GATC
PflMI 321 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
525 CCACTTCGTGG
PstI 391 CTGCAG CTGCA^G
497 CTGCAG
Sau3AI 384 GATC ^GATC
594 GATC
StyI 614 CCATGG C^C[AT][AT]GG
629 CCATGG
TaqI 29 TCGA T^CGA
365 TCGA
TthHB8I 29 TCGA T^CGA
365 TCGA
Van91I 321 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
525 CCACTTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI ApaI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII ItaI
MaeIII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
SfcI TseI Tsp45I TspRI