Restriction map for
pF1KE2525
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BbrPI BbvCI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRV FseI
FspI HindIII HpaI KpnI MaeI
MamI MluI MroI NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
ScaI SfiI SfuI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1075 GTAGAC GT^[AC][GT]AC
AcyI 178 GGCGCC G[GA]^CG[CT]C
ApaI 159 GGGCCC GGGCC^C
Asp700I 1288 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 900 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 178 GGCGCC GGCGC^C
BcgI 252 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 178 GGCGCC G[GA]^CG[CT]C
BseRI 838 GAGGAG GAGGAG(10/8)
BsmI 462 GAATGC GAATGC(1/-1)
BspMI 715 GCAGGT ACCTGC(4/8)
BsrDI 723 CATTGC GCAATG(2/0)
BsrFI 28 GCCGGC [GA]^CCGG[CT]
BssSI 528 CACGAG CACGAG(-5/-1)
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
DrdI 1513 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 367 ATGCAT ATGCA^T
HgaI 701 GCGTC GACGC(5/10)
Hin1I 178 GGCGCC G[GA]^CG[CT]C
HincII 976 GTTGAC GT[CT]^[GA]AC
HindII 976 GTTGAC GT[CT]^[GA]AC
KasI 178 GGCGCC G^GCGCC
KspI 98 CCGCGG CCGC^GG
MaeII 1097 ACGT A^CGT
MfeI 414 CAATTG C^AATTG
MunI 414 CAATTG C^AATTG
NaeI 28 GCCGGC GCC^GGC
NarI 178 GGCGCC GG^CGCC
NcoI 128 CCATGG C^CATGG
NdeI 1432 CATATG CA^TATG
NgoMIV 28 GCCGGC G^CCGGC
NsiI 367 ATGCAT ATGCA^T
PflFI 900 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 285 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1274 GACTC GAGTC(4/5)
PshAI 1057 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 98 CCGCGG CCGC^GG
SbfI 134 CCTGCAGG CCTGCA^GG
SexAI 200 ACCTGGT A^CC[AT]GGT
SfoI 178 GGCGCC GGC^GCC
SgrAI 27 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 134 CCTGCAGG CCTGCA^GG
TaqI 1185 TCGA T^CGA
Tsp45I 1555 GTCAC ^GT[GC]AC
Tth111I 900 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1185 TCGA T^CGA
Van91I 285 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1288 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BalI 230 TGGCCA TGG^CCA
513 TGGCCA
BamHI 743 GGATCC G^GATCC
1427 GGATCC
BbsI 1320 GAAGAC GAAGAC(2/6)
1243 GTCTTC
BclI 372 TGATCA T^GATCA
818 TGATCA
BpmI 1148 CTCCAG CTGGAG(16/14)
844 CTGGAG
BpuAI 1320 GAAGAC GAAGAC(2/6)
1243 GTCTTC
BsgI 831 CTGCAC GTGCAG(16/14)
713 GTGCAG
BsiEI 248 CGGCCG CG[GA][CT]^CG
693 CGGCCG
BspHI 621 TCATGA T^CATGA
956 TCATGA
BstAPI 824 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1156 GCAATAGCTGC
EagI 248 CGGCCG C^GGCCG
693 CGGCCG
EclXI 248 CGGCCG C^GGCCG
693 CGGCCG
Eco52I 248 CGGCCG C^GGCCG
693 CGGCCG
EcoRI 361 GAATTC G^AATTC
1081 GAATTC
EcoT14I 128 CCATGG C^C[AT][AT]GG
1016 CCAAGG
FbaI 372 TGATCA T^GATCA
818 TGATCA
HaeII 25 AGCGCC [GA]GCGC^[CT]
178 GGCGCC
MluNI 230 TGGCCA TGG^CCA
513 TGGCCA
MscI 230 TGGCCA TGG^CCA
513 TGGCCA
MseI 428 TTAA T^TAA
893 TTAA
PpuMI 1004 GGGTCCC [GA]G^G[AT]CC[CT]
1019 AGGACCT
PvuII 347 CAGCTG CAG^CTG
676 CAGCTG
RcaI 621 TCATGA T^CATGA
956 TCATGA
SapI 1546 GAAGAGC GCTCTTC(1/4)
1138 GCTCTTC
SfaNI 442 GATGC GCATC(5/9)
1106 GCATC
StyI 128 CCATGG C^C[AT][AT]GG
1016 CCAAGG
Tru9I 428 TTAA T^TAA
893 TTAA
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TfiI TseI Tsp509I TspRI
XcmI XhoII