Restriction map for
pF1KE2435
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NarI NcoI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 820 CGCG CG^CG
AciI 21 CCGC CCGC(-3/-1)
AcsI 117 GAATTT [GA]^AATT[CT]
AfaI 47 GTAC GT^AC
AlwNI 142 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 117 GAATTT [GA]^AATT[CT]
BanII 941 GGGCTC G[GA]GC[CT]^C
BbvCI 336 CCTCAGC CCTCAGC(-5/-2)
BcnI 986 CCCGG CC^[GC]GG
BsaI 405 GGTCTC GGTCTC(1/5)
BseRI 912 GAGGAG GAGGAG(10/8)
BsgI 459 CTGCAC GTGCAG(16/14)
BsmFI 866 GTCCC GGGAC(10/14)
BspHI 524 TCATGA T^CATGA
BsrDI 899 GCAATG GCAATG(2/0)
BsrFI 188 GCCGGC [GA]^CCGG[CT]
BsrI 548 ACTGG ACTGG(1/-1)
BssSI 701 CTCGTG CACGAG(-5/-1)
BstAPI 201 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 820 CGCG CG^CG
CfoI 819 GCGC GCG^C
Cfr10I 188 GCCGGC [GA]^CCGG[CT]
Cfr13I 720 GGCCC G^G[ACGT]CC
EcoRV 771 GATATC GAT^ATC
HaeIII 720 GGCC GG^CC
HhaI 819 GCGC GCG^C
HinP1I 819 GCGC G^CGC
HincII 3 GTTGAC GT[CT]^[GA]AC
HindII 3 GTTGAC GT[CT]^[GA]AC
MvnI 820 CGCG CG^CG
NaeI 188 GCCGGC GCC^GGC
NciI 986 CCCGG CC^[GC]GG
NgoMIV 188 GCCGGC G^CCGGC
NspI 876 GCATGC [GA]CATG^[CT]
PflMI 314 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 6 GACTC GAGTC(4/5)
RcaI 524 TCATGA T^CATGA
RsaI 47 GTAC GT^AC
Sau96I 720 GGCCC G^G[ACGT]CC
SmlI 736 CTCAAG C^T[CT][GA]AG
SphI 876 GCATGC GCATG^C
Van91I 314 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BglII 416 AGATCT A^GATCT
561 AGATCT
BmyI 799 GTGCCC G[AGT]GC[ACT]^C
941 GGGCTC
Bsp1286I 799 GTGCCC G[AGT]GC[ACT]^C
941 GGGCTC
BstYI 416 AGATCT [GA]^GATC[CT]
561 AGATCT
EarI 586 CTCTTC CTCTTC(1/4)
135 GAAGAG
HapII 189 CCGG C^CGG
987 CCGG
HpaII 189 CCGG C^CGG
987 CCGG
HphI 114 GGTGA GGTGA(8/7)
834 GGTGA
Ksp632I 586 CTCTTC CTCTTC(1/4)
135 GAAGAG
MaeIII 508 GTCAC ^GT[ACGT]AC
704 GTGAC
MflI 416 AGATCT [GA]^GATC[CT]
561 AGATCT
MseI 557 TTAA T^TAA
728 TTAA
MspI 189 CCGG C^CGG
987 CCGG
TfiI 810 GATTC G^A[AT]TC
982 GATTC
Tru9I 557 TTAA T^TAA
728 TTAA
Tsp45I 508 GTCAC ^GT[GC]AC
704 GTGAC
Tsp509I 118 AATT ^AATT
568 AATT
XhoII 416 AGATCT [GA]^GATC[CT]
561 AGATCT
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HinfI ItaI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI TseI
TspRI