Restriction map for
pF1KE2570
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 9 CGCG CG^CG
AcsI 632 AAATTT [GA]^AATT[CT]
AfaI 1176 GTAC GT^AC
AhdI 738 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 632 AAATTT [GA]^AATT[CT]
AspEI 738 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 593 TGGCCA TGG^CCA
BbsI 1564 GAAGAC GAAGAC(2/6)
BciVI 253 GTATCC GTATCC(6/5)
BlpI 286 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC
BpuAI 1564 GAAGAC GAAGAC(2/6)
BsaWI 1254 ACCGGA [AT]^CCGG[AT]
BsmI 96 GAATGC GAATGC(1/-1)
BspHI 912 TCATGA T^CATGA
BsrBI 1180 GAGCGG CCGCTC(-3/-3)
BssHII 8 GCGCGC G^CGCGC
BstEII 1420 GGTTACC G^GT[ACGT]ACC
BstPI 1420 GGTTACC G^GT[ACGT]ACC
BstUI 9 CGCG CG^CG
BstYI 939 GGATCT [GA]^GATC[CT]
CelII 286 GCTCAGC GC^T[ACGT]AGC
EaeI 593 TGGCCA [CT]^GGCC[GA]
Eam1105I 738 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1458 CTCTTC CTCTTC(1/4)
EcoO65I 1420 GGTTACC G^GT[ACGT]ACC
HgaI 582 GACGC GACGC(5/10)
Ksp632I 1458 CTCTTC CTCTTC(1/4)
MflI 939 GGATCT [GA]^GATC[CT]
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
MvnI 9 CGCG CG^CG
NspI 1280 GCATGC [GA]CATG^[CT]
PshAI 1122 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1402 CTGCAG CTGCA^G
PvuII 1512 CAGCTG CAG^CTG
RcaI 912 TCATGA T^CATGA
RsaI 1176 GTAC GT^AC
SacI 846 GAGCTC GAGCT^C
SmaI 684 CCCGGG CCC^GGG
SmlI 943 CTTGAG C^T[CT][GA]AG
SphI 1280 GCATGC GCATG^C
XhoII 939 GGATCT [GA]^GATC[CT]
XmaCI 684 CCCGGG C^CCGGG
XmaI 684 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 939 GGATC GGATC(4/5)
1517 GGATC
AvaI 609 CTCGGG C^[CT]CG[GA]G
684 CCCGGG
BfaI 323 CTAG C^TAG
1237 CTAG
BsaI 838 GAGACC GGTCTC(1/5)
1453 GAGACC
BsoBI 609 CTCGGG C^[CT]CG[GA]G
684 CCCGGG
BssSI 798 CACGAG CACGAG(-5/-1)
1327 CTCGTG
DraIII 490 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
956 CACAGAGTG
DsaI 317 CCGTGG C^C[GA][CT]GG
1124 CCGTGG
EcoNI 1536 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1551 CCTGGATGAGG
HincII 42 GTCAAC GT[CT]^[GA]AC
307 GTCAAC
HindII 42 GTCAAC GT[CT]^[GA]AC
307 GTCAAC
MaeI 323 CTAG C^TAG
1237 CTAG
TaqI 359 TCGA T^CGA
580 TCGA
Tsp509I 633 AATT ^AATT
1028 AATT
TthHB8I 359 TCGA T^CGA
580 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI XcmI