Restriction map for
pF1KE2757
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AviII BbrPI BfrI BseAI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FspI
HgaI HpaI KspI MroI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SphI
Sse8387I SwaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 3400 GGTACC G^GTACC
AccI 5180 GTAGAC GT^[AC][GT]AC
AccII 1170 CGCG CG^CG
AcyI 1692 GGCGCC G[GA]^CG[CT]C
Asp718I 3400 GGTACC G^GTACC
AvrII 3250 CCTAGG C^CTAGG
BbeI 1692 GGCGCC GGCGC^C
BglI 2549 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3807 AGATCT A^GATCT
BlnI 3250 CCTAGG C^CTAGG
BlpI 150 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaAI 1038 TACGTA [CT]AC^GT[GA]
BsaHI 1692 GGCGCC G[GA]^CG[CT]C
BsaI 3622 GAGACC GGTCTC(1/5)
BsaWI 1444 ACCGGA [AT]^CCGG[AT]
BseRI 3918 CTCCTC GAGGAG(10/8)
BsiEI 1113 CGACCG CG[GA][CT]^CG
BsmBI 1708 GAGACG CGTCTC(1/5)
Bsp1407I 2043 TGTACA T^GTACA
BspHI 3741 TCATGA T^CATGA
BsrBI 1144 CCGCTC CCGCTC(-3/-3)
BsrFI 1258 GCCGGC [GA]^CCGG[CT]
BsrGI 2043 TGTACA T^GTACA
BstUI 1170 CGCG CG^CG
BstXI 699 CCACACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 150 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1258 GCCGGC [GA]^CCGG[CT]
DraI 597 TTTAAA TTT^AAA
EcoRI 3502 GAATTC G^AATTC
EcoRV 4099 GATATC GAT^ATC
EcoT22I 3372 ATGCAT ATGCA^T
FseI 1257 GGCCGGCC GGCCGG^CC
Hin1I 1692 GGCGCC G[GA]^CG[CT]C
HincII 1831 GTCAAC GT[CT]^[GA]AC
HindII 1831 GTCAAC GT[CT]^[GA]AC
HindIII 4651 AAGCTT A^AGCTT
KasI 1692 GGCGCC G^GCGCC
KpnI 3400 GGTACC GGTAC^C
MluI 1169 ACGCGT A^CGCGT
MvnI 1170 CGCG CG^CG
NaeI 1258 GCCGGC GCC^GGC
NarI 1692 GGCGCC GG^CGCC
NgoMIV 1258 GCCGGC G^CCGGC
NheI 811 GCTAGC G^CTAGC
NsiI 3372 ATGCAT ATGCA^T
RcaI 3741 TCATGA T^CATGA
SacI 2796 GAGCTC GAGCT^C
SexAI 3021 ACCAGGT A^CC[AT]GGT
SfoI 1692 GGCGCC GGC^GCC
SnaBI 1038 TACGTA TAC^GTA
SpeI 3982 ACTAGT A^CTAGT
SspBI 2043 TGTACA T^GTACA
StuI 3171 AGGCCT AGG^CCT
XbaI 220 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 431 AACGTT AA^CGTT
3323 AACGTT
AocI 675 CCTGAGG CC^T[ACGT]AGG
1244 CCTTAGG
AseI 3973 ATTAAT AT^TAAT
3994 ATTAAT
AsnI 3973 ATTAAT AT^TAAT
3994 ATTAAT
AspI 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3130 GACCCTGTC
AvaI 2241 CCCGGG C^[CT]CG[GA]G
3485 CCCGGG
BamHI 609 GGATCC G^GATCC
2332 GGATCC
BsaBI 2182 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5167 GATTTGAATC
BsgI 4179 CTGCAC GTGCAG(16/14)
993 GTGCAG
BsoBI 2241 CCCGGG C^[CT]CG[GA]G
3485 CCCGGG
BspLU11I 1587 ACATGT A^CATGT
2106 ACATGT
BspMI 3052 ACCTGC ACCTGC(4/8)
3397 GCAGGT
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
1244 CCTTAGG
EarI 1129 CTCTTC CTCTTC(1/4)
4842 GAAGAG
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
1244 CCTTAGG
Ksp632I 1129 CTCTTC CTCTTC(1/4)
4842 GAAGAG
MamI 2182 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5167 GATTTGAATC
MfeI 2640 CAATTG C^AATTG
2814 CAATTG
MunI 2640 CAATTG C^AATTG
2814 CAATTG
PflFI 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3130 GACCCTGTC
PshBI 3973 ATTAAT AT^TAAT
3994 ATTAAT
Psp1406I 431 AACGTT AA^CGTT
3323 AACGTT
SmaI 2241 CCCGGG CCC^GGG
3485 CCCGGG
Tth111I 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3130 GACCCTGTC
XcmI 1337 CCACCGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4965 CCAACACCAGGCTGG
XmaCI 2241 CCCGGG C^CCGGG
3485 CCCGGG
XmaI 2241 CCCGGG C^CCGGG
3485 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI