Restriction map for
pF1KB4546
##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AccIII AscI AvrII BbeI
BlnI BseAI BsiWI BsmBI BspEI
BsrBI Bst1107I BstZ17I CpoI DrdI
EagI EclXI Eco52I EcoRV FseI
HpaI KasI MluI MroI NarI
NotI NruI PacI PmeI PvuI
RsrII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AclI 6076 AACGTT AA^CGTT
AflII 8598 CTTAAG C^TTAAG
AhdI 4648 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 6549 ATTAAT AT^TAAT
AsnI 6549 ATTAAT AT^TAAT
AspEI 4648 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 6629 TGCGCA TGC^GCA
BbvCI 60 GCTGAGG CCTCAGC(-5/-2)
BfrI 8598 CTTAAG C^TTAAG
BlpI 4371 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4371 GCTCAGC GC^T[ACGT]AGC
BsiEI 9725 CGACCG CG[GA][CT]^CG
BstBI 6517 TTCGAA TT^CGAA
CelII 4371 GCTCAGC GC^T[ACGT]AGC
Eam1105I 4648 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 6629 TGCGCA TGC^GCA
KspI 6104 CCGCGG CCGC^GG
NheI 3501 GCTAGC G^CTAGC
NspV 6517 TTCGAA TT^CGAA
PshAI 10355 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6549 ATTAAT AT^TAAT
Psp1406I 6076 AACGTT AA^CGTT
SacII 6104 CCGCGG CCGC^GG
SfuI 6517 TTCGAA TT^CGAA
StuI 762 AGGCCT AGG^CCT
XbaI 5559 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 3158 GGTACC G^GTACC
6320 GGTACC
AgeI 1405 ACCGGT A^CCGGT
9727 ACCGGT
Aor51HI 39 AGCGCT AGC^GCT
8048 AGCGCT
Asp718I 3158 GGTACC G^GTACC
6320 GGTACC
BcgI 8766 CGAGGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4629 GCATTTGCTTCG
BglI 1021 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4759 GCCCAGTGGGC
BssHII 67 GCGCGC G^CGCGC
69 GCGCGC
BstEII 361 GGTAACC G^GT[ACGT]ACC
9400 GGTGACC
BstPI 361 GGTAACC G^GT[ACGT]ACC
9400 GGTGACC
DraI 5521 TTTAAA TTT^AAA
9364 TTTAAA
DraIII 8745 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
10057 CACGCTGTG
Eco47III 39 AGCGCT AGC^GCT
8048 AGCGCT
EcoO65I 361 GGTAACC G^GT[ACGT]ACC
9400 GGTGACC
EcoRI 2507 GAATTC G^AATTC
8756 GAATTC
EcoT22I 703 ATGCAT ATGCA^T
7470 ATGCAT
HindIII 2139 AAGCTT A^AGCTT
8475 AAGCTT
KpnI 3158 GGTACC GGTAC^C
6320 GGTACC
NaeI 6340 GCCGGC GCC^GGC
8065 GCCGGC
NdeI 734 CATATG CA^TATG
3138 CATATG
NgoMIV 6340 GCCGGC G^CCGGC
8065 GCCGGC
NsiI 703 ATGCAT ATGCA^T
7470 ATGCAT
PaeR7I 3869 CTCGAG C^TCGAG
9974 CTCGAG
PinAI 1405 ACCGGT A^CCGGT
9727 ACCGGT
SacI 542 GAGCTC GAGCT^C
7571 GAGCTC
SspI 6946 AATATT AAT^ATT
7790 AATATT
XhoI 3869 CTCGAG C^TCGAG
9974 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvI BciVI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI