Restriction map for
pF1KB4543
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
AocI Aor51HI ApoI AseI AsnI
Asp718I AvrII BamHI BbrPI BbsI
BcgI BclI BfrI BglII BlnI
BpuAI BsaAI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrDI
BsrGI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SalI ScaI
SexAI SfiI SfuI SmlI SnaBI
SpeI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3254 GACGTC GACGT^C
AhdI 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 253 GGGCCC GGGCC^C
AscI 244 GGCGCGCC GG^CGCGCC
AspEI 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2914 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2424 GCTGAGG CCTCAGC(-5/-2)
BciVI 2311 GGATAC GTATCC(6/5)
BlpI 2304 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2304 GCTCAGC GC^T[ACGT]AGC
BsaBI 879 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 332 TCCGGT [AT]^CCGG[AT]
BstAPI 1527 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2304 GCTCAGC GC^T[ACGT]AGC
EagI 71 CGGCCG C^GGCCG
Eam1105I 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
EcoNI 267 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 147 CCGCGG CCGC^GG
MamI 879 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 827 CCATGG C^CATGG
PflFI 2914 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 963 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 147 CCGCGG CCGC^GG
SgrAI 85 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2400 GCATGC GCATG^C
SspI 1887 AATATT AAT^ATT
StuI 1458 AGGCCT AGG^CCT
Tsp509I 1010 AATT ^AATT
Tth111I 2914 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 963 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3159 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 820 AACGTT AA^CGTT
1663 AACGTT
Alw44I 289 GTGCAC G^TGCAC
954 GTGCAC
ApaLI 289 GTGCAC G^TGCAC
954 GTGCAC
AviII 536 TGCGCA TGC^GCA
1232 TGCGCA
BbeI 9 GGCGCC GGCGC^C
58 GGCGCC
BsiEI 71 CGGCCG CG[GA][CT]^CG
470 CGACCG
BspHI 800 TCATGA T^CATGA
1580 TCATGA
BspLU11I 674 ACATGT A^CATGT
1779 ACATGT
BssHII 218 GCGCGC G^CGCGC
245 GCGCGC
BssSI 316 CTCGTG CACGAG(-5/-1)
565 CTCGTG
BstEII 321 GGTAACC G^GT[ACGT]ACC
504 GGTCACC
BstPI 321 GGTAACC G^GT[ACGT]ACC
504 GGTCACC
BstYI 1173 GGATCT [GA]^GATC[CT]
1562 AGATCC
EcoO65I 321 GGTAACC G^GT[ACGT]ACC
504 GGTCACC
FspI 536 TGCGCA TGC^GCA
1232 TGCGCA
KasI 9 GGCGCC G^GCGCC
58 GGCGCC
MflI 1173 GGATCT [GA]^GATC[CT]
1562 AGATCC
MseI 1615 TTAA T^TAA
2044 TTAA
NaeI 86 GCCGGC GCC^GGC
2523 GCCGGC
NarI 9 GGCGCC GG^CGCC
58 GGCGCC
NgoMIV 86 GCCGGC G^CCGGC
2523 GCCGGC
NheI 2220 GCTAGC G^CTAGC
2793 GCTAGC
PleI 1124 GACTC GAGTC(4/5)
2173 GAGTC
PpuMI 1208 GGGTCCT [GA]G^G[AT]CC[CT]
3196 AGGTCCC
Psp1406I 820 AACGTT AA^CGTT
1663 AACGTT
RcaI 800 TCATGA T^CATGA
1580 TCATGA
SbfI 339 CCTGCAGG CCTGCA^GG
729 CCTGCAGG
SfoI 9 GGCGCC GGC^GCC
58 GGCGCC
Sse8387I 339 CCTGCAGG CCTGCA^GG
729 CCTGCAGG
Tru9I 1615 TTAA T^TAA
2044 TTAA
XcmI 1142 CCATCACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2108 CCAACAGTTCCCTGG
XhoII 1173 GGATCT [GA]^GATC[CT]
1562 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI XmnI