Restriction map for
pF1KB9134
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BciVI BfrI
BlnI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I CpoI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 3657 AACGTT AA^CGTT
AflIII 1600 ACACGT A^C[GA][CT]GT
AhdI 591 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 591 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3298 GCTGAGG CCTCAGC(-5/-2)
BcnI 5 CCCGG CC^[GC]GG
BglII 292 AGATCT A^GATCT
BlpI 2440 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2440 GCTAAGC GC^T[ACGT]AGC
BsaBI 1072 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2662 GAGGAG GAGGAG(10/8)
BsgI 398 GTGCAG GTGCAG(16/14)
BspDI 3877 ATCGAT AT^CGAT
CelII 2440 GCTAAGC GC^T[ACGT]AGC
CfoI 2286 GCGC GCG^C
ClaI 3877 ATCGAT AT^CGAT
DraI 331 TTTAAA TTT^AAA
DraII 1546 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 591 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1546 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 2628 GATATC GAT^ATC
FauI 118 GCGGG CCCGC(4/6)
HaeII 2285 AGCGCC [GA]GCGC^[CT]
HapII 6 CCGG C^CGG
HhaI 2286 GCGC GCG^C
HinP1I 2286 GCGC G^CGC
HpaI 699 GTTAAC GTT^AAC
HpaII 6 CCGG C^CGG
MamI 1072 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1675 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1060 CAGCTG C[AC]G^C[GT]G
MspI 6 CCGG C^CGG
NciI 5 CCCGG CC^[GC]GG
NspI 3738 ACATGC [GA]CATG^[CT]
PpuMI 1546 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3657 AACGTT AA^CGTT
PstI 2260 CTGCAG CTGCA^G
PvuII 1060 CAGCTG CAG^CTG
SapI 1855 GAAGAGC GCTCTTC(1/4)
SspI 2857 AATATT AAT^ATT
Tsp45I 3443 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 967 GTAGAC GT^[AC][GT]AC
1055 GTCTAC
BbsI 1729 GAAGAC GAAGAC(2/6)
1876 GAAGAC
BcgI 3592 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1065 GCAAGCAGATCG
BclI 419 TGATCA T^GATCA
672 TGATCA
BglI 1969 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2199 GCCCATGAGGC
BpuAI 1729 GAAGAC GAAGAC(2/6)
1876 GAAGAC
BsaAI 1601 CACGTA [CT]AC^GT[GA]
3440 TACGTG
BsmI 2965 GAATGC GAATGC(1/-1)
23 GCATTC
Bsp1407I 198 TGTACA T^GTACA
2702 TGTACA
BspHI 3008 TCATGA T^CATGA
3804 TCATGA
BsrDI 3681 CATTGC GCAATG(2/0)
1484 GCAATG
BsrGI 198 TGTACA T^GTACA
2702 TGTACA
BstAPI 1222 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3144 GCAGGTTTTGC
FbaI 419 TGATCA T^GATCA
672 TGATCA
HaeIII 491 GGCC GG^CC
3645 GGCC
HindIII 656 AAGCTT A^AGCTT
2551 AAGCTT
PflMI 1566 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3786 CCAGCTTCTGG
RcaI 3008 TCATGA T^CATGA
3804 TCATGA
SacI 387 GAGCTC GAGCT^C
1438 GAGCTC
SmlI 218 CTCAAG C^T[CT][GA]AG
2134 CTCAAG
SspBI 198 TGTACA T^GTACA
2702 TGTACA
Van91I 1566 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3786 CCAGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI