Restriction map for
pF1KE2754
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI HaeII HapII HgaI
Hin1I HindIII HpaI HpaII KasI
Ksp632I KspI MfeI MflI MluI
MluNI MroI MscI MseI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 770 GGTACC G^GTACC
AflIII 540 ACATGT A^C[GA][CT]GT
Asp718I 770 GGTACC G^GTACC
AviII 407 TGCGCA TGC^GCA
AvrII 120 CCTAGG C^CTAGG
BanI 770 GGTACC G^G[CT][GA]CC
BcgI 23 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 121 CTAG C^TAG
BlnI 120 CCTAGG C^CTAGG
BsaAI 341 CACGTA [CT]AC^GT[GA]
BsaBI 58 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 85 CTCCTC GAGGAG(10/8)
BsmFI 153 GGGAC GGGAC(10/14)
Bsp1407I 537 TGTACA T^GTACA
BspLU11I 540 ACATGT A^CATGT
BsrDI 199 GCAATG GCAATG(2/0)
BsrGI 537 TGTACA T^GTACA
BssKI 470 CCTGG ^CC[ACGT]GG
BstEII 317 GGTCACC G^GT[ACGT]ACC
BstNI 470 CCTGG CC^[AT]GG
BstPI 317 GGTCACC G^GT[ACGT]ACC
CfoI 408 GCGC GCG^C
DsaI 463 CCGTGG C^C[GA][CT]GG
EcoO65I 317 GGTCACC G^GT[ACGT]ACC
EcoRII 470 CCTGG ^CC[AT]GG
FspI 407 TGCGCA TGC^GCA
HhaI 408 GCGC GCG^C
HinP1I 408 GCGC G^CGC
HincII 304 GTTGAC GT[CT]^[GA]AC
HindII 304 GTTGAC GT[CT]^[GA]AC
HphI 319 TCACC GGTGA(8/7)
KpnI 770 GGTACC GGTAC^C
MaeI 121 CTAG C^TAG
MamI 58 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 76 GAAGA GAAGA(8/7)
MspA1I 533 CAGCTG C[AC]G^C[GT]G
MvaI 470 CCTGG CC^[AT]GG
NspI 540 ACATGT [GA]CATG^[CT]
PleI 482 GAGTC GAGTC(4/5)
PstI 494 CTGCAG CTGCA^G
PvuII 533 CAGCTG CAG^CTG
ScaI 225 AGTACT AGT^ACT
ScrFI 470 CCTGG CC^[ACGT]GG
SexAI 469 ACCTGGT A^CC[AT]GGT
SfcI 494 CTGCAG C^T[GA][CT]AG
SmlI 190 CTCAAG C^T[CT][GA]AG
SspBI 537 TGTACA T^GTACA
SspI 614 AATATT AAT^ATT
TfiI 125 GATTC G^A[AT]TC
TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 370 AAATTT [GA]^AATT[CT]
487 AAATTT
AlwI 64 GATCC GGATC(4/5)
50 GGATC
ApoI 370 AAATTT [GA]^AATT[CT]
487 AAATTT
AvaII 154 GGACC G^G[AT]CC
467 GGACC
BbvI 493 GCTGC GCAGC(8/12)
750 GCTGC
BspMI 346 ACCTGC ACCTGC(4/8)
496 GCAGGT
EcoT14I 120 CCTAGG C^C[AT][AT]GG
149 CCTTGG
Fnu4HI 493 GCTGC GC^[ACGT]GC
750 GCTGC
HaeIII 3 GGCC GG^CC
581 GGCC
HinfI 125 GATTC G^A[ACGT]TC
482 GAGTC
ItaI 493 GCTGC GC^[ACGT]GC
750 GCTGC
MaeII 208 ACGT A^CGT
342 ACGT
StyI 120 CCTAGG C^C[AT][AT]GG
149 CCTTGG
TaqI 22 TCGA T^CGA
291 TCGA
TseI 493 GCTGC G^C[AT]GC
750 GCTGC
TthHB8I 22 TCGA T^CGA
291 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI AluI BsaJI BsrI BstF5I
Cac8I Cfr13I DpnII FokI MaeIII
MboI MnlI MslI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfaNI
Tsp45I Tsp509I