Restriction map for
pF1KB5828
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BbsI BbvCI BciVI BclI BfrI
BlnI BpmI BpuAI BsaAI BsaBI
BsaWI BseAI BsmBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI DraI DraIII DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SalI ScaI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI Tsp509I TspRI XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Alw44I 310 GTGCAC G^TGCAC
AlwNI 28 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 609 AGCGCT AGC^GCT
ApaI 1033 GGGCCC GGGCC^C
ApaLI 310 GTGCAC G^TGCAC
AscI 214 GGCGCGCC GG^CGCGCC
AspI 1318 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 488 TGGCCA TGG^CCA
BamHI 1415 GGATCC G^GATCC
BcgI 166 GCAGTCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 944 AGATCT A^GATCT
BlpI 888 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 888 GCTGAGC GC^T[ACGT]AGC
BseRI 661 GAGGAG GAGGAG(10/8)
BsgI 1220 CTGCAC GTGCAG(16/14)
BsiWI 206 CGTACG C^GTACG
BsmI 1132 GCATTC GAATGC(1/-1)
Bsp1407I 1088 TGTACA T^GTACA
BsrGI 1088 TGTACA T^GTACA
BssSI 969 CACGAG CACGAG(-5/-1)
BstF5I 729 GGATG GGATG(2/0)
BstXI 597 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 888 GCTGAGC GC^T[ACGT]AGC
CpoI 1286 CGGTCCG CG^G[AT]CCG
EagI 89 CGGCCG C^GGCCG
EclXI 89 CGGCCG C^GGCCG
Eco47III 609 AGCGCT AGC^GCT
Eco52I 89 CGGCCG C^GGCCG
FokI 729 GGATG GGATG(9/13)
MaeII 818 ACGT A^CGT
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MseI 1354 TTAA T^TAA
NcoI 1203 CCATGG C^CATGG
NotI 88 GCGGCCGC GC^GGCCGC
PflFI 1318 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 941 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 835 GACTC GAGTC(4/5)
PpuMI 377 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 13 CAGCTG CAG^CTG
RsrII 1286 CGGTCCG CG^G[AT]CCG
SapI 612 GCTCTTC GCTCTTC(1/4)
SbfI 915 CCTGCAGG CCTGCA^GG
SexAI 526 ACCTGGT A^CC[AT]GGT
SfaNI 680 GCATC GCATC(5/9)
SgrAI 544 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 915 CCTGCAGG CCTGCA^GG
SspBI 1088 TGTACA T^GTACA
Tru9I 1354 TTAA T^TAA
Tth111I 1318 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 941 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 14 AGCT AG^CT
140 AGCT
BfaI 1076 CTAG C^TAG
1420 CTAG
BsaI 1316 GAGACC GGTCTC(1/5)
519 GGTCTC
BspMI 1093 ACCTGC ACCTGC(4/8)
516 GCAGGT
BsrI 1118 ACTGG ACTGG(1/-1)
363 CCAGT
EarI 262 CTCTTC CTCTTC(1/4)
613 CTCTTC
Ksp632I 262 CTCTTC CTCTTC(1/4)
613 CTCTTC
MaeI 1076 CTAG C^TAG
1420 CTAG
MaeIII 169 GTCAC ^GT[ACGT]AC
571 GTGAC
MslI 171 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CACCAAGATG
NspI 185 GCATGT [GA]CATG^[CT]
1160 ACATGC
StuI 428 AGGCCT AGG^CCT
1280 AGGCCT
TfiI 453 GAATC G^A[AT]TC
1336 GATTC
Tsp45I 169 GTCAC ^GT[GC]AC
571 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfcI SfoI SmaI SmlI StyI
TaqI TseI TthHB8I XhoII XmaCI
XmaI