Restriction map for
pF1KE2747
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FseI FspI HpaI KspI
MluI MroI MvnI NaeI NciI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1154 GGTACC G^GTACC
AccI 1247 GTCGAC GT^[AC][GT]AC
AcyI 2442 GGCGCC G[GA]^CG[CT]C
AflIII 858 ACATGT A^C[GA][CT]GT
Alw44I 980 GTGCAC G^TGCAC
ApaLI 980 GTGCAC G^TGCAC
Asp718I 1154 GGTACC G^GTACC
AspHI 980 GTGCAC G[AT]GC[AT]^C
BalI 1592 TGGCCA TGG^CCA
BanII 2271 GAGCCC G[GA]GC[CT]^C
BbeI 2442 GGCGCC GGCGC^C
BbvCI 950 CCTCAGC CCTCAGC(-5/-2)
BclI 1364 TGATCA T^GATCA
BpmI 1802 CTGGAG CTGGAG(16/14)
BsaBI 407 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2442 GGCGCC G[GA]^CG[CT]C
BsiHKAI 980 GTGCAC G[AT]GC[AT]^C
Bsp1407I 638 TGTACA T^GTACA
BspLU11I 858 ACATGT A^CATGT
BspMI 94 GCAGGT ACCTGC(4/8)
BsrDI 441 CATTGC GCAATG(2/0)
BsrGI 638 TGTACA T^GTACA
BstAPI 1228 GCAAATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2252 TTCGAA TT^CGAA
CfoI 2443 GCGC GCG^C
DraI 1525 TTTAAA TTT^AAA
EcoRV 2471 GATATC GAT^ATC
FauI 2510 CCCGC CCCGC(4/6)
FbaI 1364 TGATCA T^GATCA
HaeII 2442 GGCGCC [GA]GCGC^[CT]
HgaI 1250 GACGC GACGC(5/10)
HhaI 2443 GCGC GCG^C
Hin1I 2442 GGCGCC G[GA]^CG[CT]C
HinP1I 2443 GCGC G^CGC
HincII 1247 GTCGAC GT[CT]^[GA]AC
HindII 1247 GTCGAC GT[CT]^[GA]AC
HindIII 2248 AAGCTT A^AGCTT
KasI 2442 GGCGCC G^GCGCC
KpnI 1154 GGTACC GGTAC^C
MamI 407 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1592 TGGCCA TGG^CCA
MscI 1592 TGGCCA TGG^CCA
MslI 2295 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2442 GGCGCC GG^CGCC
NspV 2252 TTCGAA TT^CGAA
PaeR7I 2410 CTCGAG C^TCGAG
PleI 4 GAGTC GAGTC(4/5)
SalI 1247 GTCGAC G^TCGAC
SfoI 2442 GGCGCC GGC^GCC
SfuI 2252 TTCGAA TT^CGAA
SphI 1741 GCATGC GCATG^C
SspBI 638 TGTACA T^GTACA
SspI 1231 AATATT AAT^ATT
XhoI 2410 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 229 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1165 GAACTGTTTC
AvaI 2189 CTCGGG C^[CT]CG[GA]G
2410 CTCGAG
BanI 1154 GGTACC G^G[CT][GA]CC
2442 GGCGCC
BciVI 1912 GGATAC GTATCC(6/5)
1275 GTATCC
BglI 2445 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2489 GCCCAGACGGC
BsaI 1974 GAGACC GGTCTC(1/5)
2129 GAGACC
BseRI 992 GAGGAG GAGGAG(10/8)
2545 GAGGAG
BsmI 1434 GAATGC GAATGC(1/-1)
2258 GCATTC
BsoBI 2189 CTCGGG C^[CT]CG[GA]G
2410 CTCGAG
BspHI 623 TCATGA T^CATGA
1841 TCATGA
BstYI 216 GGATCT [GA]^GATC[CT]
523 GGATCT
DraII 606 GGGTCCT [GA]G^G[ACGT]CC[CT]
846 AGGTCCC
DsaI 1150 CCATGG C^C[GA][CT]GG
1724 CCATGG
EcoO109I 606 GGGTCCT [GA]G^G[ACGT]CC[CT]
846 AGGTCCC
EcoRI 719 GAATTC G^AATTC
1939 GAATTC
HapII 428 CCGG C^CGG
2479 CCGG
HpaII 428 CCGG C^CGG
2479 CCGG
HphI 145 GGTGA GGTGA(8/7)
1653 TCACC
MaeII 1967 ACGT A^CGT
2429 ACGT
MfeI 986 CAATTG C^AATTG
2138 CAATTG
MflI 216 GGATCT [GA]^GATC[CT]
523 GGATCT
MspI 428 CCGG C^CGG
2479 CCGG
MunI 986 CAATTG C^AATTG
2138 CAATTG
NcoI 1150 CCATGG C^CATGG
1724 CCATGG
NspI 858 ACATGT [GA]CATG^[CT]
1741 GCATGC
PflMI 238 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1847 CCAGTAAATGG
PpuMI 606 GGGTCCT [GA]G^G[AT]CC[CT]
846 AGGTCCC
PshAI 1250 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1384 GACTGTGGTC
RcaI 623 TCATGA T^CATGA
1841 TCATGA
SmlI 654 CTTGAG C^T[CT][GA]AG
2410 CTCGAG
Van91I 238 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1847 CCAGTAAATGG
XhoII 216 GGATCT [GA]^GATC[CT]
523 GGATCT
XmnI 229 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1165 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I