Restriction map for
pF1KE6622
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BlnI BmyI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I TspRI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 133 AACGTT AA^CGTT
AlwI 507 GGATC GGATC(4/5)
Aor51HI 224 AGCGCT AGC^GCT
AvaI 703 CCCGAG C^[CT]CG[GA]G
BglI 786 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 195 AGATCT A^GATCT
BlpI 175 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 175 GCTAAGC GC^T[ACGT]AGC
BsmAI 300 GAGAC GTCTC(1/5)
BsmBI 300 GAGACG CGTCTC(1/5)
BsmI 308 GCATTC GAATGC(1/-1)
BsoBI 703 CCCGAG C^[CT]CG[GA]G
BspHI 809 TCATGA T^CATGA
BsrI 21 CCAGT ACTGG(1/-1)
BstAPI 406 GCAACCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 324 GGATG GGATG(2/0)
BstYI 195 AGATCT [GA]^GATC[CT]
CelII 175 GCTAAGC GC^T[ACGT]AGC
EaeI 793 CGGCCA [CT]^GGCC[GA]
EarI 367 GAAGAG CTCTTC(1/4)
Eco47III 224 AGCGCT AGC^GCT
EcoT14I 659 CCAAGG C^C[AT][AT]GG
FauI 666 GCGGG CCCGC(4/6)
FokI 324 GGATG GGATG(9/13)
HaeII 224 AGCGCT [GA]GCGC^[CT]
HgaI 305 GACGC GACGC(5/10)
HindIII 644 AAGCTT A^AGCTT
Ksp632I 367 GAAGAG CTCTTC(1/4)
MaeII 134 ACGT A^CGT
MflI 195 AGATCT [GA]^GATC[CT]
MspA1I 139 CAGCGG C[AC]G^C[GT]G
NlaIV 143 GGAACC GG[ACGT]^[ACGT]CC
PflMI 78 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 133 AACGTT AA^CGTT
PstI 97 CTGCAG CTGCA^G
RcaI 809 TCATGA T^CATGA
SbfI 96 CCTGCAGG CCTGCA^GG
SfcI 97 CTGCAG C^T[GA][CT]AG
SfiI 785 GGCCAGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 96 CCTGCAGG CCTGCA^GG
StyI 659 CCAAGG C^C[AT][AT]GG
Van91I 78 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 195 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 269 AAATTC [GA]^AATT[CT]
735 AAATTC
ApoI 269 AAATTC [GA]^AATT[CT]
735 AAATTC
AvaII 75 GGACC G^G[AT]CC
153 GGTCC
BsiYI 78 CCACACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
659 CCAAGGAGCGG
BslI 78 CCACACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
659 CCAAGGAGCGG
BsrBI 587 CCGCTC CCGCTC(-3/-3)
664 GAGCGG
CfoI 205 GCGC GCG^C
225 GCGC
HhaI 205 GCGC GCG^C
225 GCGC
HinP1I 205 GCGC G^CGC
225 GCGC
HinfI 38 GAGTC G^A[ACGT]TC
886 GAGTC
HphI 820 GGTGA GGTGA(8/7)
765 TCACC
MaeIII 360 GTTAC ^GT[ACGT]AC
428 GTTAC
PleI 38 GAGTC GAGTC(4/5)
886 GAGTC
SmlI 590 CTCAAG C^T[CT][GA]AG
599 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TthHB8I