Restriction map for
pF1KE2661
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspEI BspHI BspLU11I BspMI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 682 GTCGAC GT^[AC][GT]AC
AcyI 1842 GACGCC G[GA]^CG[CT]C
AfaI 696 GTAC GT^AC
AlwNI 1221 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1565 GTGCTC G[AT]GC[AT]^C
BglI 1880 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 1565 GTGCTC G[AGT]GC[ACT]^C
BpmI 155 CTGGAG CTGGAG(16/14)
BsaHI 1842 GACGCC G[GA]^CG[CT]C
BsaI 1871 GAGACC GGTCTC(1/5)
BsgI 676 CTGCAC GTGCAG(16/14)
BsiHKAI 1565 GTGCTC G[AT]GC[AT]^C
Bsp1286I 1565 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 695 TGTACA T^GTACA
BspDI 131 ATCGAT AT^CGAT
BsrBI 59 GAGCGG CCGCTC(-3/-3)
BsrGI 695 TGTACA T^GTACA
BssSI 1402 CTCGTG CACGAG(-5/-1)
ClaI 131 ATCGAT AT^CGAT
DraI 121 TTTAAA TTT^AAA
DraII 1502 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 349 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1502 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 776 CCAAGG C^C[AT][AT]GG
HgaI 1842 GACGC GACGC(5/10)
Hin1I 1842 GACGCC G[GA]^CG[CT]C
MslI 1299 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 988 GCCGGC GCC^GGC
NgoMIV 988 GCCGGC G^CCGGC
NspI 567 ACATGC [GA]CATG^[CT]
PflMI 816 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1025 GAGTC GAGTC(4/5)
PpuMI 1502 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1794 CTGCAG CTGCA^G
PvuII 577 CAGCTG CAG^CTG
RsaI 696 GTAC GT^AC
SalI 682 GTCGAC G^TCGAC
SapI 1572 GCTCTTC GCTCTTC(1/4)
SspBI 695 TGTACA T^GTACA
StyI 776 CCAAGG C^C[AT][AT]GG
Van91I 816 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 576 CCAGCTGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BbsI 76 GTCTTC GAAGAC(2/6)
1059 GTCTTC
BpuAI 76 GTCTTC GAAGAC(2/6)
1059 GTCTTC
BsaBI 34 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1486 GATGAAAATC
BsmFI 186 GGGAC GGGAC(10/14)
773 GTCCC
BsmI 1735 GAATGC GAATGC(1/-1)
808 GCATTC
BsrDI 163 GCAATG GCAATG(2/0)
859 GCAATG
BsrFI 937 ACCGGC [GA]^CCGG[CT]
988 GCCGGC
BstAPI 793 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1558 GCAGAGAGTGC
Cfr10I 937 ACCGGC [GA]^CCGG[CT]
988 GCCGGC
EcoT22I 791 ATGCAT ATGCA^T
1737 ATGCAT
HapII 938 CCGG C^CGG
989 CCGG
HindIII 1829 AAGCTT A^AGCTT
1900 AAGCTT
HpaII 938 CCGG C^CGG
989 CCGG
HphI 1789 TCACC GGTGA(8/7)
1834 TCACC
MamI 34 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1486 GATGAAAATC
MspA1I 577 CAGCTG C[AC]G^C[GT]G
1639 CCGCTG
MspI 938 CCGG C^CGG
989 CCGG
NlaIV 1195 GGAGCC GG[ACGT]^[ACGT]CC
1503 GGACCC
NsiI 791 ATGCAT ATGCA^T
1737 ATGCAT
TaqI 132 TCGA T^CGA
683 TCGA
Tsp45I 649 GTGAC ^GT[GC]AC
1027 GTCAC
TthHB8I 132 TCGA T^CGA
683 TCGA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BalI BbvI BfaI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI XhoII