Restriction map for
pF1KE2662
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 370 GTCTAC GT^[AC][GT]AC
AfaI 495 GTAC GT^AC
AflIII 470 ACATGT A^C[GA][CT]GT
AlwI 315 GATCC GGATC(4/5)
AvaI 679 CCCGGG C^[CT]CG[GA]G
AvaII 736 GGACC G^G[AT]CC
BalI 536 TGGCCA TGG^CCA
BbsI 118 GTCTTC GAAGAC(2/6)
BglI 271 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 377 AGATCT A^GATCT
BpuAI 118 GTCTTC GAAGAC(2/6)
BsaI 457 GAGACC GGTCTC(1/5)
BseRI 619 GAGGAG GAGGAG(10/8)
BsiEI 281 CGGCCG CG[GA][CT]^CG
BsoBI 679 CCCGGG C^[CT]CG[GA]G
BspLU11I 470 ACATGT A^CATGT
BsrFI 596 ACCGGC [GA]^CCGG[CT]
BsrI 134 ACTGG ACTGG(1/-1)
BssSI 34 CACGAG CACGAG(-5/-1)
BstYI 377 AGATCT [GA]^GATC[CT]
Cfr10I 596 ACCGGC [GA]^CCGG[CT]
DraII 735 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 281 CGGCCG C^GGCCG
EarI 181 CTCTTC CTCTTC(1/4)
EclXI 281 CGGCCG C^GGCCG
Eco52I 281 CGGCCG C^GGCCG
EcoNI 609 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 735 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 305 CCATGG C^C[AT][AT]GG
HaeII 268 AGCGCC [GA]GCGC^[CT]
HinfI 704 GAGTC G^A[ACGT]TC
Ksp632I 181 CTCTTC CTCTTC(1/4)
MaeII 110 ACGT A^CGT
MflI 377 AGATCT [GA]^GATC[CT]
MluNI 536 TGGCCA TGG^CCA
MscI 536 TGGCCA TGG^CCA
MspA1I 201 CAGCGG C[AC]G^C[GT]G
NcoI 305 CCATGG C^CATGG
NotI 280 GCGGCCGC GC^GGCCGC
PleI 704 GAGTC GAGTC(4/5)
PpuMI 735 GGGACCC [GA]G^G[AT]CC[CT]
PstI 613 CTGCAG CTGCA^G
RsaI 495 GTAC GT^AC
ScaI 494 AGTACT AGT^ACT
SmaI 679 CCCGGG CCC^GGG
SmlI 625 CTCAAG C^T[CT][GA]AG
StuI 17 AGGCCT AGG^CCT
StyI 305 CCATGG C^C[AT][AT]GG
TspRI 515 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 101 CCATCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 377 AGATCT [GA]^GATC[CT]
XmaCI 679 CCCGGG C^CCGGG
XmaI 679 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccII 56 CGCG CG^CG
217 CGCG
BsmAI 457 GAGAC GTCTC(1/5)
691 GTCTC
BstAPI 39 GCACTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
326 GCATCACCTGC
BstUI 56 CGCG CG^CG
217 CGCG
DsaI 305 CCATGG C^C[GA][CT]GG
719 CCGTGG
EaeI 281 CGGCCG [CT]^GGCC[GA]
536 TGGCCA
MslI 63 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
105 CATCAACGTG
MvnI 56 CGCG CG^CG
217 CGCG
NspI 44 ACATGC [GA]CATG^[CT]
470 ACATGT
SacI 220 GAGCTC GAGCT^C
622 GAGCTC
SfcI 613 CTGCAG C^T[GA][CT]AG
656 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI