Restriction map for
pF1KE2472
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI AviII AvrII BbrPI BbvCI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
FseI FspI HincII HindII HpaI
MfeI MluI MroI MunI NciI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2426 GGTACC G^GTACC
AcyI 42 GGCGCC G[GA]^CG[CT]C
AflIII 2869 ACGTGT A^C[GA][CT]GT
AhdI 1999 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2009 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2592 AGCGCT AGC^GCT
Asp718I 2426 GGTACC G^GTACC
AspEI 1999 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4073 GGATCC G^GATCC
BbeI 42 GGCGCC GGCGC^C
BcgI 160 GCAGGTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3336 GTATCC GTATCC(6/5)
BglI 1776 GCCAGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3331 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GGCGCC G[GA]^CG[CT]C
BsaI 3131 GGTCTC GGTCTC(1/5)
BstBI 1598 TTCGAA TT^CGAA
BstYI 4073 GGATCC [GA]^GATC[CT]
DraI 3769 TTTAAA TTT^AAA
DrdI 1363 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1999 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2592 AGCGCT AGC^GCT
EcoRI 2569 GAATTC G^AATTC
Hin1I 42 GGCGCC G[GA]^CG[CT]C
KasI 42 GGCGCC G^GCGCC
KpnI 2426 GGTACC GGTAC^C
KspI 46 CCGCGG CCGC^GG
MamI 3331 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4073 GGATCC [GA]^GATC[CT]
NaeI 2236 GCCGGC GCC^GGC
NarI 42 GGCGCC GG^CGCC
NcoI 234 CCATGG C^CATGG
NgoMIV 2236 GCCGGC G^CCGGC
NspV 1598 TTCGAA TT^CGAA
PshAI 2988 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 46 CCGCGG CCGC^GG
ScaI 380 AGTACT AGT^ACT
SfoI 42 GGCGCC GGC^GCC
SfuI 1598 TTCGAA TT^CGAA
SphI 2955 GCATGC GCATG^C
TaqI 1599 TCGA T^CGA
TthHB8I 1599 TCGA T^CGA
XhoII 4073 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 47 CGCG CG^CG
1832 CGCG
AseI 1236 ATTAAT AT^TAAT
3385 ATTAAT
AsnI 1236 ATTAAT AT^TAAT
3385 ATTAAT
Asp700I 2050 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4351 GAAAATATTC
AspI 1588 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4178 GACCAAGTC
AvaI 85 CCCGAG C^[CT]CG[GA]G
1792 CCCGAG
BbsI 895 GAAGAC GAAGAC(2/6)
3253 GAAGAC
BpuAI 895 GAAGAC GAAGAC(2/6)
3253 GAAGAC
BseRI 1023 GAGGAG GAGGAG(10/8)
3601 GAGGAG
BsoBI 85 CCCGAG C^[CT]CG[GA]G
1792 CCCGAG
BsrBI 928 CCGCTC CCGCTC(-3/-3)
88 GAGCGG
BsrDI 2345 CATTGC GCAATG(2/0)
1915 GCAATG
BstUI 47 CGCG CG^CG
1832 CGCG
DraII 704 AGGACCT [GA]G^G[ACGT]CC[CT]
2986 GGGACCC
EcoO109I 704 AGGACCT [GA]G^G[ACGT]CC[CT]
2986 GGGACCC
FauI 372 CCCGC CCCGC(4/6)
48 GCGGG
MaeII 2373 ACGT A^CGT
2869 ACGT
MvnI 47 CGCG CG^CG
1832 CGCG
NspI 209 ACATGC [GA]CATG^[CT]
2955 GCATGC
PflFI 1588 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4178 GACCAAGTC
PpuMI 704 AGGACCT [GA]G^G[AT]CC[CT]
2986 GGGACCC
PshBI 1236 ATTAAT AT^TAAT
3385 ATTAAT
PvuII 886 CAGCTG CAG^CTG
1426 CAGCTG
SacI 2108 GAGCTC GAGCT^C
2158 GAGCTC
SapI 3901 GAAGAGC GCTCTTC(1/4)
1735 GCTCTTC
Tth111I 1588 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4178 GACCAAGTC
XcmI 1128 CCACTGTGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1873 CCACTTGGTCGCTGG
XmnI 2050 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4351 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvI BclI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI PflMI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I