Restriction map for
pF1KE1860
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AlwNI 1114 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 731 CCTCAGG CC^T[ACGT]AGG
AspHI 448 GAGCAC G[AT]GC[AT]^C
BalI 317 TGGCCA TGG^CCA
BbsI 966 GTCTTC GAAGAC(2/6)
BbvCI 1119 GCTGAGG CCTCAGC(-5/-2)
BfaI 328 CTAG C^TAG
BglII 110 AGATCT A^GATCT
BpuAI 966 GTCTTC GAAGAC(2/6)
BsiHKAI 448 GAGCAC G[AT]GC[AT]^C
BsmBI 7 GAGACG CGTCTC(1/5)
BsrFI 310 GCCGGC [GA]^CCGG[CT]
BstAPI 682 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 975 GGTGACC G^GT[ACGT]ACC
BstPI 975 GGTGACC G^GT[ACGT]ACC
BstYI 110 AGATCT [GA]^GATC[CT]
Bsu36I 731 CCTCAGG CC^T[ACGT]AGG
Cfr10I 310 GCCGGC [GA]^CCGG[CT]
DpnII 111 GATC ^GATC
DsaI 125 CCACGG C^C[GA][CT]GG
Eco81I 731 CCTCAGG CC^T[ACGT]AGG
EcoO65I 975 GGTGACC G^GT[ACGT]ACC
HaeII 458 AGCGCC [GA]GCGC^[CT]
MaeI 328 CTAG C^TAG
MaeIII 976 GTGAC ^GT[ACGT]AC
MboI 111 GATC ^GATC
MflI 110 AGATCT [GA]^GATC[CT]
MluNI 317 TGGCCA TGG^CCA
MscI 317 TGGCCA TGG^CCA
MslI 33 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 310 GCCGGC GCC^GGC
NdeII 111 GATC ^GATC
NgoMIV 310 GCCGGC G^CCGGC
PflMI 171 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 584 CTGCAG CTGCA^G
PvuII 581 CAGCTG CAG^CTG
Sau3AI 111 GATC ^GATC
SexAI 376 ACCTGGT A^CC[AT]GGT
TaqI 768 TCGA T^CGA
Tsp45I 976 GTGAC ^GT[GC]AC
TthHB8I 768 TCGA T^CGA
Van91I 171 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 110 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BsgI 617 CTGCAC GTGCAG(16/14)
999 CTGCAC
BspMI 486 ACCTGC ACCTGC(4/8)
174 GCAGGT
EarI 793 GAAGAG CTCTTC(1/4)
1057 GAAGAG
EcoNI 532 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
947 CCTTGCCAAGG
EcoT14I 854 CCAAGG C^C[AT][AT]GG
952 CCAAGG
FauI 496 CCCGC CCCGC(4/6)
1128 GCGGG
Ksp632I 793 GAAGAG CTCTTC(1/4)
1057 GAAGAG
MspA1I 497 CCGCTG C[AC]G^C[GT]G
581 CAGCTG
PleI 785 GACTC GAGTC(4/5)
893 GAGTC
SfcI 584 CTGCAG C^T[GA][CT]AG
1030 CTACAG
StyI 854 CCAAGG C^C[AT][AT]GG
952 CCAAGG
TfiI 862 GAATC G^A[AT]TC
910 GAATC
Tsp509I 133 AATT ^AATT
1073 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PpuMI Sau96I ScrFI
SfaNI StuI TseI TspRI