Restriction map for
pF1KE2700
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI AspEI AspHI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KspI MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1290 GGTACC G^GTACC
AccIII 1418 TCCGGA T^CCGGA
AocI 355 CCTGAGG CC^T[ACGT]AGG
Aor51HI 724 AGCGCT AGC^GCT
ApaI 716 GGGCCC GGGCC^C
Asp700I 1479 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1290 GGTACC G^GTACC
AvaI 1242 CCCGGG C^[CT]CG[GA]G
BalI 1383 TGGCCA TGG^CCA
BsaI 420 GAGACC GGTCTC(1/5)
BsaWI 1418 TCCGGA [AT]^CCGG[AT]
BseAI 1418 TCCGGA T^CCGGA
BsoBI 1242 CCCGGG C^[CT]CG[GA]G
BspEI 1418 TCCGGA T^CCGGA
BspHI 299 TCATGA T^CATGA
BspMI 1196 ACCTGC ACCTGC(4/8)
BssSI 1045 CACGAG CACGAG(-5/-1)
BstAPI 382 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 753 CCAGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
Eco47III 724 AGCGCT AGC^GCT
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
FauI 261 CCCGC CCCGC(4/6)
HaeII 724 AGCGCT [GA]GCGC^[CT]
HincII 1276 GTCAAC GT[CT]^[GA]AC
HindII 1276 GTCAAC GT[CT]^[GA]AC
KpnI 1290 GGTACC GGTAC^C
MaeII 896 ACGT A^CGT
MluNI 1383 TGGCCA TGG^CCA
MroI 1418 TCCGGA T^CCGGA
MscI 1383 TGGCCA TGG^CCA
MslI 654 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1450 CAGCTG C[AC]G^C[GT]G
PpuMI 1338 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1450 CAGCTG CAG^CTG
RcaI 299 TCATGA T^CATGA
ScaI 1085 AGTACT AGT^ACT
SexAI 1341 ACCTGGT A^CC[AT]GGT
SmaI 1242 CCCGGG CCC^GGG
TfiI 87 GAATC G^A[AT]TC
Tsp509I 565 AATT ^AATT
XcmI 84 CCAGAATCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1242 CCCGGG C^CCGGG
XmaI 1242 CCCGGG C^CCGGG
XmnI 1479 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 14 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCCTGTC
BbsI 1102 GTCTTC GAAGAC(2/6)
1237 GTCTTC
BfaI 835 CTAG C^TAG
1099 CTAG
BpuAI 1102 GTCTTC GAAGAC(2/6)
1237 GTCTTC
BseRI 313 GAGGAG GAGGAG(10/8)
688 GAGGAG
BsmFI 116 GGGAC GGGAC(10/14)
640 GGGAC
BsrBI 43 GAGCGG CCGCTC(-3/-3)
220 GAGCGG
BsrDI 123 CATTGC GCAATG(2/0)
1232 GCAATG
BstYI 443 AGATCC [GA]^GATC[CT]
1502 GGATCT
DraII 324 GGGGCCT [GA]G^G[ACGT]CC[CT]
1338 AGGACCT
DrdI 642 GACAGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1267 GACTCTGAGGTC
EarI 1357 CTCTTC CTCTTC(1/4)
160 GAAGAG
EcoO109I 324 GGGGCCT [GA]G^G[ACGT]CC[CT]
1338 AGGACCT
Ksp632I 1357 CTCTTC CTCTTC(1/4)
160 GAAGAG
MaeI 835 CTAG C^TAG
1099 CTAG
MflI 443 AGATCC [GA]^GATC[CT]
1502 GGATCT
PflFI 14 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCCTGTC
PflMI 1176 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1398 CCAGTCCTTGG
PshAI 979 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1132 GACTTCTGTC
PstI 380 CTGCAG CTGCA^G
1000 CTGCAG
TaqI 584 TCGA T^CGA
767 TCGA
Tth111I 14 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCCTGTC
TthHB8I 584 TCGA T^CGA
767 TCGA
Van91I 1176 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1398 CCAGTCCTTGG
XhoII 443 AGATCC [GA]^GATC[CT]
1502 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI