Restriction map for
pF1KB9620
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AvrII BalI BamHI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
Eam1105I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NdeII
NheI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SapI Sau3AI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 577 GTCTAC GT^[AC][GT]AC
AfaI 1038 GTAC GT^AC
Aor51HI 180 AGCGCT AGC^GCT
ApaI 502 GGGCCC GGGCC^C
AviII 827 TGCGCA TGC^GCA
BbsI 900 GAAGAC GAAGAC(2/6)
BbvCI 984 CCTCAGC CCTCAGC(-5/-2)
BpuAI 900 GAAGAC GAAGAC(2/6)
BseRI 394 GAGGAG GAGGAG(10/8)
BsmFI 78 GTCCC GGGAC(10/14)
BsrBI 33 GAGCGG CCGCTC(-3/-3)
BsrFI 121 GCCGGC [GA]^CCGG[CT]
BssHII 219 GCGCGC G^CGCGC
BstAPI 1023 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 339 GGTCACC G^GT[ACGT]ACC
BstF5I 321 CATCC GGATG(2/0)
BstPI 339 GGTCACC G^GT[ACGT]ACC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
DraIII 1024 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 889 GAAGAG CTCTTC(1/4)
Eco47III 180 AGCGCT AGC^GCT
EcoO65I 339 GGTCACC G^GT[ACGT]ACC
EcoT14I 377 CCAAGG C^C[AT][AT]GG
FokI 321 CATCC GGATG(9/13)
FspI 827 TGCGCA TGC^GCA
HincII 994 GTCAAC GT[CT]^[GA]AC
HindII 994 GTCAAC GT[CT]^[GA]AC
HinfI 912 GAGTC G^A[ACGT]TC
HphI 341 TCACC GGTGA(8/7)
Ksp632I 889 GAAGAG CTCTTC(1/4)
KspI 116 CCGCGG CCGC^GG
MaeIII 340 GTCAC ^GT[ACGT]AC
MslI 780 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 121 GCCGGC GCC^GGC
NgoMIV 121 GCCGGC G^CCGGC
PaeR7I 307 CTCGAG C^TCGAG
PleI 912 GAGTC GAGTC(4/5)
PvuII 1009 CAGCTG CAG^CTG
RsaI 1038 GTAC GT^AC
SacII 116 CCGCGG CCGC^GG
SexAI 1025 ACCAGGT A^CC[AT]GGT
StyI 377 CCAAGG C^C[AT][AT]GG
Tsp45I 340 GTCAC ^GT[GC]AC
TspRI 54 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 307 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 999 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1009 CAGCTGCTG
AspHI 304 GAGCTC G[AT]GC[AT]^C
357 GAGCTC
BcgI 941 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
830 GCAACCGCATCG
BglI 38 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
547 GCCCCGGCGGC
BlpI 30 GCTGAGC GC^T[ACGT]AGC
153 GCTGAGC
BpmI 430 CTGGAG CTGGAG(16/14)
868 CTGGAG
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
153 GCTGAGC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
357 GAGCTC
BspMI 168 GCAGGT ACCTGC(4/8)
957 GCAGGT
BsrI 53 CCAGT ACTGG(1/-1)
324 CCAGT
BstXI 329 CCAACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCATCGACATGG
CelII 30 GCTGAGC GC^T[ACGT]AGC
153 GCTGAGC
MaeII 743 ACGT A^CGT
992 ACGT
NlaIII 129 CATG CATG^
786 CATG
SacI 304 GAGCTC GAGCT^C
357 GAGCTC
SmaI 81 CCCGGG CCC^GGG
97 CCCGGG
TaqI 308 TCGA T^CGA
782 TCGA
TthHB8I 308 TCGA T^CGA
782 TCGA
XmaCI 81 CCCGGG C^CCGGG
97 CCCGGG
XmaI 81 CCCGGG C^CCGGG
97 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsoBI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIV
NotI Sau96I ScrFI SfaNI SfoI
SmlI TseI