Restriction map for
pF1KE9279
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BsaI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MroI MseI
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 838 CGCG CG^CG
AlwNI 721 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 51 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 69 CCCGAG C^[CT]CG[GA]G
BalI 230 TGGCCA TGG^CCA
BbrPI 706 CACGTG CAC^GTG
BbsI 532 GTCTTC GAAGAC(2/6)
BpuAI 532 GTCTTC GAAGAC(2/6)
BsaAI 706 CACGTG [CT]AC^GT[GA]
BsaWI 458 ACCGGA [AT]^CCGG[AT]
BsiEI 834 CGGCCG CG[GA][CT]^CG
BsoBI 69 CCCGAG C^[CT]CG[GA]G
BspMI 33 GCAGGT ACCTGC(4/8)
BsrFI 832 GCCGGC [GA]^CCGG[CT]
BstF5I 6 CATCC GGATG(2/0)
BstUI 838 CGCG CG^CG
CfoI 396 GCGC GCG^C
Cfr10I 832 GCCGGC [GA]^CCGG[CT]
DraIII 171 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 834 CGGCCG C^GGCCG
EclXI 834 CGGCCG C^GGCCG
Eco52I 834 CGGCCG C^GGCCG
FokI 6 CATCC GGATG(9/13)
HaeII 395 GGCGCT [GA]GCGC^[CT]
HgaI 75 GACGC GACGC(5/10)
HhaI 396 GCGC GCG^C
HinP1I 396 GCGC G^CGC
MluNI 230 TGGCCA TGG^CCA
MscI 230 TGGCCA TGG^CCA
MvnI 838 CGCG CG^CG
NaeI 832 GCCGGC GCC^GGC
NgoMIV 832 GCCGGC G^CCGGC
NspI 315 GCATGT [GA]CATG^[CT]
PmaCI 706 CACGTG CAC^GTG
PmlI 706 CACGTG CAC^GTG
PstI 187 CTGCAG CTGCA^G
SapI 21 GAAGAGC GCTCTTC(1/4)
SfcI 187 CTGCAG C^T[GA][CT]AG
TaqI 668 TCGA T^CGA
Tsp509I 452 AATT ^AATT
TthHB8I 668 TCGA T^CGA
XmnI 51 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 216 GTAC GT^AC
678 GTAC
BseRI 121 CTCCTC GAGGAG(10/8)
43 GAGGAG
BsmAI 821 GAGAC GTCTC(1/5)
196 GTCTC
DdeI 263 CTGAG C^T[ACGT]AG
623 CTGAG
EaeI 230 TGGCCA [CT]^GGCC[GA]
834 CGGCCG
EarI 124 CTCTTC CTCTTC(1/4)
21 GAAGAG
FauI 652 CCCGC CCCGC(4/6)
29 GCGGG
Ksp632I 124 CTCTTC CTCTTC(1/4)
21 GAAGAG
MspA1I 105 CAGCTG C[AC]G^C[GT]G
466 CAGCTG
MwoI 389 GCCCTGGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
796 GCCAACATCGC
PflMI 81 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
412 CCAGATAATGG
PvuII 105 CAGCTG CAG^CTG
466 CAGCTG
RsaI 216 GTAC GT^AC
678 GTAC
TfiI 249 GATTC G^A[AT]TC
627 GAATC
Tsp45I 293 GTGAC ^GT[GC]AC
751 GTGAC
Van91I 81 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
412 CCAGATAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MslI MspI MvaI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TseI TspRI