Restriction map for
pF1KE3496
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# NO CUT ENZYMES ( 48 enzymes) #
##################################
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BbvCI
BcgI BlnI BspDI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DraI Eco47III EcoO65I EcoT22I FseI
HpaI KspI MluI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SfuI SgrAI SnaBI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1654 GACGTC GACGT^C
Acc65I 557 GGTACC G^GTACC
AclI 3492 AACGTT AA^CGTT
AocI 505 CCTGAGG CC^T[ACGT]AGG
ApaI 2783 GGGCCC GGGCC^C
Asp718I 557 GGTACC G^GTACC
BbrPI 286 CACGTG CAC^GTG
BlpI 2826 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2826 GCTGAGC GC^T[ACGT]AGC
BsaAI 286 CACGTG [CT]AC^GT[GA]
BsiEI 4394 CGGCCG CG[GA][CT]^CG
BsiWI 4062 CGTACG C^GTACG
Bsp1407I 3920 TGTACA T^GTACA
BsrGI 3920 TGTACA T^GTACA
Bst1107I 5556 GTATAC GTA^TAC
BstZ17I 5556 GTATAC GTA^TAC
Bsu36I 505 CCTGAGG CC^T[ACGT]AGG
CelII 2826 GCTGAGC GC^T[ACGT]AGC
EagI 4394 CGGCCG C^GGCCG
EclXI 4394 CGGCCG C^GGCCG
Eco52I 4394 CGGCCG C^GGCCG
Eco81I 505 CCTGAGG CC^T[ACGT]AGG
EcoRI 3979 GAATTC G^AATTC
EcoRV 382 GATATC GAT^ATC
HindIII 157 AAGCTT A^AGCTT
KpnI 557 GGTACC GGTAC^C
MfeI 1800 CAATTG C^AATTG
MunI 1800 CAATTG C^AATTG
NdeI 456 CATATG CA^TATG
PaeR7I 3443 CTCGAG C^TCGAG
PmaCI 286 CACGTG CAC^GTG
PmlI 286 CACGTG CAC^GTG
Psp1406I 3492 AACGTT AA^CGTT
SacI 3376 GAGCTC GAGCT^C
SapI 3117 GCTCTTC GCTCTTC(1/4)
SfiI 247 GGCCACTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 34 GCATGC GCATG^C
SspBI 3920 TGTACA T^GTACA
SspI 5597 AATATT AAT^ATT
StuI 3188 AGGCCT AGG^CCT
XhoI 3443 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 1532 CGCG CG^CG
2628 CGCG
AccIII 81 TCCGGA T^CCGGA
2314 TCCGGA
AflII 2725 CTTAAG C^TTAAG
3370 CTTAAG
AhdI 234 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4247 GACACGGAGTC
AspEI 234 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4247 GACACGGAGTC
BamHI 569 GGATCC G^GATCC
4001 GGATCC
BbeI 1243 GGCGCC GGCGC^C
4585 GGCGCC
BciVI 2572 GGATAC GTATCC(6/5)
1805 GTATCC
BclI 1904 TGATCA T^GATCA
2591 TGATCA
BfrI 2725 CTTAAG C^TTAAG
3370 CTTAAG
BglII 668 AGATCT A^GATCT
4091 AGATCT
BsaI 4359 GGTCTC GGTCTC(1/5)
4694 GGTCTC
BseAI 81 TCCGGA T^CCGGA
2314 TCCGGA
BspEI 81 TCCGGA T^CCGGA
2314 TCCGGA
BstAPI 4400 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4570 GCAACCCCTGC
BstUI 1532 CGCG CG^CG
2628 CGCG
DrdI 2545 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2920 GACAGCAAGGTC
Eam1105I 234 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4247 GACACGGAGTC
FbaI 1904 TGATCA T^GATCA
2591 TGATCA
HaeII 1243 GGCGCC [GA]GCGC^[CT]
4585 GGCGCC
KasI 1243 GGCGCC G^GCGCC
4585 GGCGCC
MroI 81 TCCGGA T^CCGGA
2314 TCCGGA
MvnI 1532 CGCG CG^CG
2628 CGCG
NarI 1243 GGCGCC GG^CGCC
4585 GGCGCC
SalI 1701 GTCGAC G^TCGAC
3089 GTCGAC
SbfI 3891 CCTGCAGG CCTGCA^GG
3990 CCTGCAGG
SfoI 1243 GGCGCC GGC^GCC
4585 GGCGCC
SmaI 3247 CCCGGG CCC^GGG
4713 CCCGGG
SpeI 1212 ACTAGT A^CTAGT
5314 ACTAGT
Sse8387I 3891 CCTGCAGG CCTGCA^GG
3990 CCTGCAGG
XmaCI 3247 CCCGGG C^CCGGG
4713 CCCGGG
XmaI 3247 CCCGGG C^CCGGG
4713 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI