Restriction map for
pF1KE2802
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 132 GTAC GT^AC
AflIII 5 ACGTGT A^C[GA][CT]GT
AluI 293 AGCT AG^CT
AlwI 18 GATCC GGATC(4/5)
AlwNI 124 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 321 CTCGGG C^[CT]CG[GA]G
BanI 23 GGCGCC G^G[CT][GA]CC
BbeI 23 GGCGCC GGCGC^C
BcgI 65 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 237 CCGGG CC^[GC]GG
BglI 232 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 349 GTGCAG GTGCAG(16/14)
BsoBI 321 CTCGGG C^[CT]CG[GA]G
Bsp1407I 131 TGTACA T^GTACA
BspHI 14 TCATGA T^CATGA
BsrGI 131 TGTACA T^GTACA
BstBI 73 TTCGAA TT^CGAA
BstNI 315 CCAGG CC^[AT]GG
DpnII 18 GATC ^GATC
EaeI 202 CGGCCA [CT]^GGCC[GA]
EcoRII 315 CCAGG ^CC[AT]GG
EcoT14I 53 CCAAGG C^C[AT][AT]GG
FauI 285 CCCGC CCCGC(4/6)
HaeII 23 GGCGCC [GA]GCGC^[CT]
HgaI 49 GACGC GACGC(5/10)
HphI 185 TCACC GGTGA(8/7)
KasI 23 GGCGCC G^GCGCC
MaeII 5 ACGT A^CGT
MboI 18 GATC ^GATC
MboII 41 TCTTC GAAGA(8/7)
MspA1I 124 CAGCGG C[AC]G^C[GT]G
MvaI 315 CCAGG CC^[AT]GG
NarI 23 GGCGCC GG^CGCC
NciI 237 CCGGG CC^[GC]GG
NdeII 18 GATC ^GATC
NlaIII 15 CATG CATG^
NspV 73 TTCGAA TT^CGAA
PflMI 144 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 14 TCATGA T^CATGA
RsaI 132 GTAC GT^AC
Sau3AI 18 GATC ^GATC
SfoI 23 GGCGCC GGC^GCC
SfuI 73 TTCGAA TT^CGAA
SmlI 103 CTCAAG C^T[CT][GA]AG
SspBI 131 TGTACA T^GTACA
StyI 53 CCAAGG C^C[AT][AT]GG
Van91I 144 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 218 CCACAGTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
BbvI 123 GCAGC GCAGC(8/12)
294 GCTGC
BsaHI 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
BsaJI 53 CCAAGG C^C[ACGT][ACGT]GG
314 CCCAGG
BsrI 168 ACTGG ACTGG(1/-1)
188 CCAGT
BssKI 237 CCGGG ^CC[ACGT]GG
315 CCAGG
Cac8I 228 GCTGGC GC[ACGT]^[ACGT]GC
284 GCCCGC
CfoI 24 GCGC GCG^C
84 GCGC
Cfr13I 210 GGCCC G^G[ACGT]CC
258 GGCCC
DraII 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
257 AGGCCCT
EcoO109I 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
257 AGGCCCT
HapII 21 CCGG C^CGG
237 CCGG
HhaI 24 GCGC GCG^C
84 GCGC
Hin1I 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
HinP1I 24 GCGC G^CGC
84 GCGC
HinfI 58 GAGTC G^A[ACGT]TC
319 GACTC
HpaII 21 CCGG C^CGG
237 CCGG
MspI 21 CCGG C^CGG
237 CCGG
NlaIV 23 GGCGCC GG[ACGT]^[ACGT]CC
210 GGCCCC
PleI 319 GACTC GAGTC(4/5)
58 GAGTC
Sau96I 210 GGCCC G^G[ACGT]CC
258 GGCCC
ScrFI 237 CCGGG CC^[ACGT]GG
315 CCAGG
TseI 123 GCAGC G^C[AT]GC
294 GCTGC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AciI BsiYI BslI BstF5I Fnu4HI
FokI HaeIII ItaI MnlI MwoI
SfaNI TaqI TspRI TthHB8I