Restriction map for
pF1KE2639
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI Aor51HI ApaI AscI AspI
AviII AvrII BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KspI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 341 GGTACC G^GTACC
AcsI 1528 AAATTC [GA]^AATT[CT]
AflIII 1592 ACATGT A^C[GA][CT]GT
AhdI 1204 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 855 GTGCAC G^TGCAC
AocI 1087 CCTCAGG CC^T[ACGT]AGG
ApaLI 855 GTGCAC G^TGCAC
ApoI 1528 AAATTC [GA]^AATT[CT]
AseI 1870 ATTAAT AT^TAAT
AsnI 1870 ATTAAT AT^TAAT
Asp700I 2001 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 341 GGTACC G^GTACC
AspEI 1204 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 218 CTCGGG C^[CT]CG[GA]G
BanII 39 GAGCTC G[GA]GC[CT]^C
BbeI 745 GGCGCC GGCGC^C
BciVI 990 GGATAC GTATCC(6/5)
BsaBI 1779 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 140 ACCGGA [AT]^CCGG[AT]
BsiEI 329 CGGCCG CG[GA][CT]^CG
BsmI 1197 GAATGC GAATGC(1/-1)
BsoBI 218 CTCGGG C^[CT]CG[GA]G
BspHI 482 TCATGA T^CATGA
BspLU11I 1592 ACATGT A^CATGT
BsrBI 1306 GAGCGG CCGCTC(-3/-3)
BssSI 1257 CACGAG CACGAG(-5/-1)
Bsu36I 1087 CCTCAGG CC^T[ACGT]AGG
DraII 1610 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 329 CGGCCG C^GGCCG
Eam1105I 1204 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 329 CGGCCG C^GGCCG
Eco52I 329 CGGCCG C^GGCCG
Eco81I 1087 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1610 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 745 GGCGCC [GA]GCGC^[CT]
HincII 1764 GTCAAC GT[CT]^[GA]AC
HindII 1764 GTCAAC GT[CT]^[GA]AC
KasI 745 GGCGCC G^GCGCC
KpnI 341 GGTACC GGTAC^C
MaeII 659 ACGT A^CGT
MamI 1779 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 193 CCGCTG C[AC]G^C[GT]G
NarI 745 GGCGCC GG^CGCC
NcoI 1407 CCATGG C^CATGG
NdeI 1055 CATATG CA^TATG
PflMI 254 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1610 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1870 ATTAAT AT^TAAT
PstI 1381 CTGCAG CTGCA^G
RcaI 482 TCATGA T^CATGA
SacI 39 GAGCTC GAGCT^C
ScaI 947 AGTACT AGT^ACT
SfcI 1381 CTGCAG C^T[GA][CT]AG
SfoI 745 GGCGCC GGC^GCC
Van91I 254 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2001 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 776 TGGCCA TGG^CCA
912 TGGCCA
BbsI 559 GAAGAC GAAGAC(2/6)
2005 GTCTTC
BcnI 102 CCGGG CC^[GC]GG
1034 CCCGG
BpuAI 559 GAAGAC GAAGAC(2/6)
2005 GTCTTC
BsaI 277 GAGACC GGTCTC(1/5)
2053 GAGACC
BsgI 604 CTGCAC GTGCAG(16/14)
1645 CTGCAC
BsrDI 692 GCAATG GCAATG(2/0)
828 GCAATG
BstXI 532 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1502 CCAGTGATGTGG
DsaI 332 CCGTGG C^C[GA][CT]GG
1407 CCATGG
EarI 1984 CTCTTC CTCTTC(1/4)
1801 GAAGAG
HgaI 1078 GACGC GACGC(5/10)
1106 GCGTC
HindIII 1243 AAGCTT A^AGCTT
1401 AAGCTT
Ksp632I 1984 CTCTTC CTCTTC(1/4)
1801 GAAGAG
MluNI 776 TGGCCA TGG^CCA
912 TGGCCA
MscI 776 TGGCCA TGG^CCA
912 TGGCCA
NciI 102 CCGGG CC^[GC]GG
1034 CCCGG
NspI 1208 GCATGT [GA]CATG^[CT]
1592 ACATGT
StuI 110 AGGCCT AGG^CCT
156 AGGCCT
Tsp45I 382 GTGAC ^GT[GC]AC
1301 GTGAC
XcmI 323 CCAAGGCGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
915 CCACGATACAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BamHI
BanI BbvI BclI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII