Restriction map for
pF1KE2658
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII BbrPI BcgI BfrI BsaAI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KspI MfeI MluI MroI MunI
MvnI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 3383 GACGTC GACGT^C
Acc65I 2080 GGTACC G^GTACC
AcsI 614 AAATTT [GA]^AATT[CT]
ApoI 614 AAATTT [GA]^AATT[CT]
Asp718I 2080 GGTACC G^GTACC
AspI 2140 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 354 CCCGGG C^[CT]CG[GA]G
AvrII 597 CCTAGG C^CTAGG
BamHI 2808 GGATCC G^GATCC
BclI 2103 TGATCA T^GATCA
BglI 1815 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1311 AGATCT A^GATCT
BlnI 597 CCTAGG C^CTAGG
BsmI 2940 GAATGC GAATGC(1/-1)
BsoBI 354 CCCGGG C^[CT]CG[GA]G
BsrBI 606 CCGCTC CCGCTC(-3/-3)
BsrFI 1034 GCCGGC [GA]^CCGG[CT]
BstEII 601 GGTGACC G^GT[ACGT]ACC
BstPI 601 GGTGACC G^GT[ACGT]ACC
Cfr10I 1034 GCCGGC [GA]^CCGG[CT]
DrdI 3352 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2087 CCATGG C^C[GA][CT]GG
EcoO65I 601 GGTGACC G^GT[ACGT]ACC
FauI 1031 CCCGC CCCGC(4/6)
FbaI 2103 TGATCA T^GATCA
HgaI 1038 GCGTC GACGC(5/10)
HindIII 530 AAGCTT A^AGCTT
KpnI 2080 GGTACC GGTAC^C
NaeI 1034 GCCGGC GCC^GGC
NcoI 2087 CCATGG C^CATGG
NgoMIV 1034 GCCGGC G^CCGGC
NspI 2581 ACATGC [GA]CATG^[CT]
PflFI 2140 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1474 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1693 CTGCAG CTGCA^G
SapI 2048 GCTCTTC GCTCTTC(1/4)
SexAI 1772 ACCAGGT A^CC[AT]GGT
SgrAI 1033 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 354 CCCGGG CCC^GGG
Tth111I 2140 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 354 CCCGGG C^CCGGG
XmaI 354 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 1069 CCTGAGG CC^T[ACGT]AGG
3125 CCTCAGG
Asp700I 1571 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2566 GAAACCCTTC
BbeI 1443 GGCGCC GGCGC^C
1555 GGCGCC
BbsI 417 GAAGAC GAAGAC(2/6)
2556 GTCTTC
BbvCI 478 CCTCAGC CCTCAGC(-5/-2)
871 GCTGAGG
BciVI 2337 GGATAC GTATCC(6/5)
1212 GTATCC
BlpI 295 GCTGAGC GC^T[ACGT]AGC
2169 GCTGAGC
Bpu1102I 295 GCTGAGC GC^T[ACGT]AGC
2169 GCTGAGC
BpuAI 417 GAAGAC GAAGAC(2/6)
2556 GTCTTC
BsaWI 33 ACCGGA [AT]^CCGG[AT]
824 ACCGGA
Bsp1407I 992 TGTACA T^GTACA
2208 TGTACA
BsrDI 23 CATTGC GCAATG(2/0)
2525 CATTGC
BsrGI 992 TGTACA T^GTACA
2208 TGTACA
BstXI 2158 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2901 CCAGGGCCTTGG
Bsu36I 1069 CCTGAGG CC^T[ACGT]AGG
3125 CCTCAGG
CelII 295 GCTGAGC GC^T[ACGT]AGC
2169 GCTGAGC
Eco81I 1069 CCTGAGG CC^T[ACGT]AGG
3125 CCTCAGG
HincII 1114 GTTGAC GT[CT]^[GA]AC
2701 GTCAAC
HindII 1114 GTTGAC GT[CT]^[GA]AC
2701 GTCAAC
KasI 1443 GGCGCC G^GCGCC
1555 GGCGCC
MaeII 626 ACGT A^CGT
3384 ACGT
MseI 1351 TTAA T^TAA
2717 TTAA
NarI 1443 GGCGCC GG^CGCC
1555 GGCGCC
PflMI 1679 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2277 CCAGGGACTGG
SacI 3224 GAGCTC GAGCT^C
3272 GAGCTC
SfcI 1693 CTGCAG C^T[GA][CT]AG
2189 CTGTAG
SfoI 1443 GGCGCC GGC^GCC
1555 GGCGCC
SspBI 992 TGTACA T^GTACA
2208 TGTACA
StuI 372 AGGCCT AGG^CCT
468 AGGCCT
TaqI 346 TCGA T^CGA
3321 TCGA
Tru9I 1351 TTAA T^TAA
2717 TTAA
TthHB8I 346 TCGA T^CGA
3321 TCGA
Van91I 1679 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2277 CCAGGGACTGG
XmnI 1571 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2566 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI TseI
Tsp45I Tsp509I TspRI XcmI XhoII