Restriction map for
pF1KE4281
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaII AviII AvrII
BbeI BbrPI BglI BlnI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr13I ClaI CpoI
DraII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 4284 GTCTAC GT^[AC][GT]AC
AspHI 1215 GAGCAC G[AT]GC[AT]^C
AvaI 3224 CCCGGG C^[CT]CG[GA]G
BbvCI 4459 CCTCAGC CCTCAGC(-5/-2)
BcgI 2585 CGATGGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3054 GTATCC GTATCC(6/5)
BlpI 168 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 168 GCTTAGC GC^T[ACGT]AGC
BsaI 4254 GAGACC GGTCTC(1/5)
BsiEI 1903 CGGCCG CG[GA][CT]^CG
BsiHKAI 1215 GAGCAC G[AT]GC[AT]^C
BsoBI 3224 CCCGGG C^[CT]CG[GA]G
BspHI 3081 TCATGA T^CATGA
BspMI 2196 ACCTGC ACCTGC(4/8)
BsrFI 1905 GCCGGT [GA]^CCGG[CT]
BstBI 3362 TTCGAA TT^CGAA
CelII 168 GCTTAGC GC^T[ACGT]AGC
CfoI 2348 GCGC GCG^C
Cfr10I 1905 GCCGGT [GA]^CCGG[CT]
DsaI 1345 CCATGG C^C[GA][CT]GG
EagI 1903 CGGCCG C^GGCCG
EclXI 1903 CGGCCG C^GGCCG
Eco52I 1903 CGGCCG C^GGCCG
EcoRV 1263 GATATC GAT^ATC
EcoT22I 483 ATGCAT ATGCA^T
FauI 3896 GCGGG CCCGC(4/6)
HhaI 2348 GCGC GCG^C
HinP1I 2348 GCGC G^CGC
HpaI 1786 GTTAAC GTT^AAC
NcoI 1345 CCATGG C^CATGG
NsiI 483 ATGCAT ATGCA^T
NspV 3362 TTCGAA TT^CGAA
PflMI 3792 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3081 TCATGA T^CATGA
SfuI 3362 TTCGAA TT^CGAA
SmaI 3224 CCCGGG CCC^GGG
SpeI 2538 ACTAGT A^CTAGT
StuI 3445 AGGCCT AGG^CCT
Van91I 3792 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3224 CCCGGG C^CCGGG
XmaI 3224 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 1627 CTTAAG C^TTAAG
1749 CTTAAG
AseI 1183 ATTAAT AT^TAAT
2091 ATTAAT
AsnI 1183 ATTAAT AT^TAAT
2091 ATTAAT
BalI 1677 TGGCCA TGG^CCA
4240 TGGCCA
BamHI 3255 GGATCC G^GATCC
4488 GGATCC
BanII 2292 GAGCCC G[GA]GC[CT]^C
3995 GAGCCC
BbsI 3569 GAAGAC GAAGAC(2/6)
4097 GTCTTC
BfrI 1627 CTTAAG C^TTAAG
1749 CTTAAG
BglII 1512 AGATCT A^GATCT
4017 AGATCT
BpmI 3790 CTCCAG CTGGAG(16/14)
3992 CTGGAG
BpuAI 3569 GAAGAC GAAGAC(2/6)
4097 GTCTTC
BsaAI 3752 CACGTA [CT]AC^GT[GA]
4159 TACGTG
BsiYI 1268 CCGAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3792 CCAGGATCTGG
BslI 1268 CCGAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3792 CCAGGATCTGG
BspLU11I 709 ACATGT A^CATGT
1666 ACATGT
Cac8I 752 GCAAGC GC[ACGT]^[ACGT]GC
2241 GCAAGC
DraI 1957 TTTAAA TTT^AAA
3174 TTTAAA
DraIII 3008 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3694 CACAAAGTG
HincII 697 GTCAAC GT[CT]^[GA]AC
1786 GTTAAC
HindII 697 GTCAAC GT[CT]^[GA]AC
1786 GTTAAC
MluNI 1677 TGGCCA TGG^CCA
4240 TGGCCA
MscI 1677 TGGCCA TGG^CCA
4240 TGGCCA
NspI 709 ACATGT [GA]CATG^[CT]
1666 ACATGT
PshBI 1183 ATTAAT AT^TAAT
2091 ATTAAT
SfcI 3501 CTATAG C^T[GA][CT]AG
3986 CTGTAG
XbaI 1056 TCTAGA T^CTAGA
2814 TCTAGA
XcmI 1336 CCAGGGCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2508 CCACTCTTCAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I BanI
BbvI BclI BcnI BfaI BmyI
BsaBI BsaJI BseRI BsgI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI DdeI DpnII EaeI EarI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI ScrFI SfaNI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI