Restriction map for
pF1KE2133
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BfrI BglI BlnI
BlpI Bpu1102I BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoT22I
FauI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MluI MluNI MroI MscI
NarI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 2040 GTGCAC G^TGCAC
ApaLI 2040 GTGCAC G^TGCAC
BcgI 1327 GCAGAAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 95 TGATCA T^GATCA
BfaI 1846 CTAG C^TAG
BsaAI 1143 TACGTG [CT]AC^GT[GA]
BsaBI 742 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1419 CTGCAC GTGCAG(16/14)
BsiEI 575 CGATCG CG[GA][CT]^CG
BsmBI 1573 GAGACG CGTCTC(1/5)
BspMI 2027 ACCTGC ACCTGC(4/8)
BssHII 13 GCGCGC G^CGCGC
BssSI 2011 CACGAG CACGAG(-5/-1)
BstAPI 713 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2387 CCAAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1596 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 2648 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 538 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1596 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2271 GAATTC G^AATTC
EcoRV 2503 GATATC GAT^ATC
FbaI 95 TGATCA T^GATCA
MaeI 1846 CTAG C^TAG
MamI 742 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1640 CAATTG C^AATTG
MslI 2757 CATTATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1640 CAATTG C^AATTG
NaeI 3 GCCGGC GCC^GGC
NdeI 2618 CATATG CA^TATG
NgoMIV 3 GCCGGC G^CCGGC
NspI 2207 GCATGC [GA]CATG^[CT]
PvuI 575 CGATCG CGAT^CG
SacI 210 GAGCTC GAGCT^C
SphI 2207 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 14 CGCG CG^CG
16 CGCG
AcyI 538 GACGCC G[GA]^CG[CT]C
1347 GGCGTC
Asp700I 2122 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2356 GAAGATCTTC
BanII 210 GAGCTC G[GA]GC[CT]^C
844 GGGCTC
BbsI 181 GAAGAC GAAGAC(2/6)
506 GAAGAC
BcnI 177 CCCGG CC^[GC]GG
2238 CCCGG
BpmI 1592 CTCCAG CTGGAG(16/14)
413 CTGGAG
BpuAI 181 GAAGAC GAAGAC(2/6)
506 GAAGAC
BsaHI 538 GACGCC G[GA]^CG[CT]C
1347 GGCGTC
BsaI 201 GAGACC GGTCTC(1/5)
2429 GAGACC
BsaWI 973 ACCGGA [AT]^CCGG[AT]
2676 TCCGGT
BsmFI 67 GGGAC GGGAC(10/14)
217 GGGAC
BsmI 718 GAATGC GAATGC(1/-1)
2672 GCATTC
BspHI 663 TCATGA T^CATGA
2507 TCATGA
BsrFI 3 GCCGGC [GA]^CCGG[CT]
1744 ACCGGC
BsrI 708 ACTGG ACTGG(1/-1)
1007 CCAGT
BstEII 1023 GGTTACC G^GT[ACGT]ACC
1740 GGTGACC
BstPI 1023 GGTTACC G^GT[ACGT]ACC
1740 GGTGACC
BstUI 14 CGCG CG^CG
16 CGCG
CfoI 13 GCGC GCG^C
15 GCGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
1744 ACCGGC
EcoO65I 1023 GGTTACC G^GT[ACGT]ACC
1740 GGTGACC
HgaI 538 GACGC GACGC(5/10)
1348 GCGTC
HhaI 13 GCGC GCG^C
15 GCGC
Hin1I 538 GACGCC G[GA]^CG[CT]C
1347 GGCGTC
HinP1I 13 GCGC G^CGC
15 GCGC
MvnI 14 CGCG CG^CG
16 CGCG
NciI 177 CCCGG CC^[GC]GG
2238 CCCGG
PaeR7I 874 CTCGAG C^TCGAG
2498 CTCGAG
RcaI 663 TCATGA T^CATGA
2507 TCATGA
ScaI 1495 AGTACT AGT^ACT
1763 AGTACT
SfcI 1726 CTACAG C^T[GA][CT]AG
2741 CTACAG
XhoI 874 CTCGAG C^TCGAG
2498 CTCGAG
XmnI 2122 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2356 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BbvI
BciVI BglII BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrDI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII