Restriction map for
pF1KE1756
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstF5I BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 65 AAATTT [GA]^AATT[CT]
AflIII 372 ACATGT A^C[GA][CT]GT
AlwNI 573 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 65 AAATTT [GA]^AATT[CT]
BbvI 622 GCTGC GCAGC(8/12)
BciVI 430 GGATAC GTATCC(6/5)
BclI 348 TGATCA T^GATCA
BcnI 592 CCCGG CC^[GC]GG
BsiYI 30 CCGATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 30 CCGATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 310 GAGAC GTCTC(1/5)
BsmBI 310 GAGACG CGTCTC(1/5)
BsmFI 588 GTCCC GGGAC(10/14)
BspLU11I 372 ACATGT A^CATGT
BstAPI 118 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 456 GGTAACC G^GT[ACGT]ACC
BstPI 456 GGTAACC G^GT[ACGT]ACC
BstYI 229 AGATCC [GA]^GATC[CT]
DdeI 616 CTCAG C^T[ACGT]AG
DsaI 744 CCATGG C^C[GA][CT]GG
EaeI 38 CGGCCA [CT]^GGCC[GA]
EcoO65I 456 GGTAACC G^GT[ACGT]ACC
EcoT14I 744 CCATGG C^C[AT][AT]GG
FauI 653 GCGGG CCCGC(4/6)
FbaI 348 TGATCA T^GATCA
HapII 593 CCGG C^CGG
HindIII 545 AAGCTT A^AGCTT
HpaII 593 CCGG C^CGG
MaeII 760 ACGT A^CGT
MflI 229 AGATCC [GA]^GATC[CT]
MspA1I 478 CAGCTG C[AC]G^C[GT]G
MspI 593 CCGG C^CGG
NciI 592 CCCGG CC^[GC]GG
NcoI 744 CCATGG C^CATGG
NspI 372 ACATGT [GA]CATG^[CT]
PvuII 478 CAGCTG CAG^CTG
SexAI 696 ACCAGGT A^CC[AT]GGT
SfaNI 212 GATGC GCATC(5/9)
StyI 744 CCATGG C^C[AT][AT]GG
TseI 622 GCTGC G^C[AT]GC
XcmI 735 CCAAGTGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 229 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 230 GATCC GGATC(4/5)
727 GATCC
BsaBI 230 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
349 GATCAGAATC
BspHI 177 TCATGA T^CATGA
194 TCATGA
BstNI 462 CCTGG CC^[AT]GG
697 CCAGG
EcoRII 462 CCTGG ^CC[AT]GG
697 CCAGG
HaeIII 39 GGCC GG^CC
387 GGCC
MamI 230 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
349 GATCAGAATC
MslI 54 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
99 CACCCAGATG
MvaI 462 CCTGG CC^[AT]GG
697 CCAGG
MwoI 118 GCACTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
734 GCCAAGTGAGC
PleI 500 GACTC GAGTC(4/5)
552 GAGTC
RcaI 177 TCATGA T^CATGA
194 TCATGA
Tsp45I 523 GTCAC ^GT[GC]AC
721 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BsaJI BseRI BsrI
BssKI BstXI Cac8I DpnII Fnu4HI
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI ScrFI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I