Restriction map for
pF1KE2142
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I AspI AvrII BamHI
BclI BfrI BglII BlnI BseAI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstYI BstZ17I ClaI CpoI
DraI EarI EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI Ksp632I MfeI MflI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SfuI SnaBI SpeI SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AhdI 1690 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2670 GATCC GGATC(4/5)
Aor51HI 755 AGCGCT AGC^GCT
AspEI 1690 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 939 CGAGCGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2698 GGATAC GTATCC(6/5)
BsaBI 1434 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1993 CGTCTC CGTCTC(1/5)
DrdI 1369 GACAACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1690 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 755 AGCGCT AGC^GCT
MamI 1434 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2322 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 1132 GCGGCCGC GC^GGCCGC
PaeR7I 536 CTCGAG C^TCGAG
SacI 1587 GAGCTC GAGCT^C
SbfI 2391 CCTGCAGG CCTGCA^GG
SexAI 732 ACCAGGT A^CC[AT]GGT
SgrAI 2049 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2391 CCTGCAGG CCTGCA^GG
TfiI 2875 GATTC G^A[AT]TC
XcmI 2675 CCATGGATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 536 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 1772 GGTACC G^GTACC
2509 GGTACC
AflIII 1562 ACACGT A^C[GA][CT]GT
2469 ACACGT
Alw44I 16 GTGCAC G^TGCAC
1744 GTGCAC
ApaLI 16 GTGCAC G^TGCAC
1744 GTGCAC
Asp718I 1772 GGTACC G^GTACC
2509 GGTACC
BbsI 2317 GAAGAC GAAGAC(2/6)
1552 GTCTTC
BfaI 463 CTAG C^TAG
1927 CTAG
BlpI 2254 GCTGAGC GC^T[ACGT]AGC
2857 GCTAAGC
Bpu1102I 2254 GCTGAGC GC^T[ACGT]AGC
2857 GCTAAGC
BpuAI 2317 GAAGAC GAAGAC(2/6)
1552 GTCTTC
BsaWI 1910 ACCGGA [AT]^CCGG[AT]
2714 ACCGGA
BspMI 1142 ACCTGC ACCTGC(4/8)
1931 ACCTGC
BsrDI 11 GCAATG GCAATG(2/0)
2276 GCAATG
BssSI 1471 CACGAG CACGAG(-5/-1)
2425 CTCGTG
BstEII 369 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
BstPI 369 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
CelII 2254 GCTGAGC GC^T[ACGT]AGC
2857 GCTAAGC
DpnII 1479 GATC ^GATC
2670 GATC
EagI 23 CGGCCG C^GGCCG
1133 CGGCCG
EclXI 23 CGGCCG C^GGCCG
1133 CGGCCG
Eco52I 23 CGGCCG C^GGCCG
1133 CGGCCG
EcoO65I 369 GGTCACC G^GT[ACGT]ACC
390 GGTGACC
KpnI 1772 GGTACC GGTAC^C
2509 GGTACC
KspI 1234 CCGCGG CCGC^GG
2825 CCGCGG
MaeI 463 CTAG C^TAG
1927 CTAG
MboI 1479 GATC ^GATC
2670 GATC
NaeI 2050 GCCGGC GCC^GGC
2074 GCCGGC
NdeII 1479 GATC ^GATC
2670 GATC
NgoMIV 2050 GCCGGC G^CCGGC
2074 GCCGGC
NspI 626 GCATGC [GA]CATG^[CT]
1653 GCATGC
PshAI 363 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1545 GACAACTGTC
SacII 1234 CCGCGG CCGC^GG
2825 CCGCGG
Sau3AI 1479 GATC ^GATC
2670 GATC
SmlI 536 CTCGAG C^T[CT][GA]AG
2638 CTTGAG
SphI 626 GCATGC GCATG^C
1653 GCATGC
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbrPI BbvCI BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DraIII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XmaCI XmaI