Restriction map for
pF1KE2463
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AvaII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FokI FseI HaeII HgaI
Hin1I HpaI HphI KasI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvaI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 414 GGTACC G^GTACC
AccI 100 GTATAC GT^[AC][GT]AC
AccII 406 CGCG CG^CG
AflIII 584 ACATGT A^C[GA][CT]GT
AlwI 532 GATCC GGATC(4/5)
AlwNI 607 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 414 GGTACC G^GTACC
AviII 560 TGCGCA TGC^GCA
BanI 414 GGTACC G^G[CT][GA]CC
BanII 22 GGGCTC G[GA]GC[CT]^C
BclI 323 TGATCA T^GATCA
BmyI 22 GGGCTC G[AGT]GC[ACT]^C
BpmI 163 CTGGAG CTGGAG(16/14)
BsaJI 363 CCAAGG C^C[ACGT][ACGT]GG
Bsp1286I 22 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 584 ACATGT A^CATGT
BspMI 33 GCAGGT ACCTGC(4/8)
Bst1107I 100 GTATAC GTA^TAC
BstUI 406 CGCG CG^CG
BstZ17I 100 GTATAC GTA^TAC
Cac8I 333 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 561 GCGC GCG^C
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoT14I 363 CCAAGG C^C[AT][AT]GG
FauI 627 GCGGG CCCGC(4/6)
FbaI 323 TGATCA T^GATCA
FspI 560 TGCGCA TGC^GCA
HhaI 561 GCGC GCG^C
HinP1I 561 GCGC G^CGC
HincII 580 GTTGAC GT[CT]^[GA]AC
HindII 580 GTTGAC GT[CT]^[GA]AC
HindIII 76 AAGCTT A^AGCTT
KpnI 414 GGTACC GGTAC^C
MaeIII 427 GTGAC ^GT[ACGT]AC
MboII 448 GAAGA GAAGA(8/7)
MslI 258 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 406 CGCG CG^CG
NspI 584 ACATGT [GA]CATG^[CT]
PleI 368 GAGTC GAGTC(4/5)
SmlI 481 CTCAAG C^T[CT][GA]AG
StuI 85 AGGCCT AGG^CCT
StyI 363 CCAAGG C^C[AT][AT]GG
TaqI 348 TCGA T^CGA
Tsp45I 427 GTGAC ^GT[GC]AC
TthHB8I 348 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 50 GTAC GT^AC
415 GTAC
BbvI 142 GCTGC GCAGC(8/12)
223 GCTGC
BcnI 20 CCGGG CC^[GC]GG
29 CCCGG
BssKI 20 CCGGG ^CC[ACGT]GG
29 CCCGG
DdeI 493 CTAAG C^T[ACGT]AG
524 CTAAG
DpnII 324 GATC ^GATC
532 GATC
DrdI 528 GACTGATCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
583 GACATGTCTGTC
HapII 20 CCGG C^CGG
30 CCGG
HpaII 20 CCGG C^CGG
30 CCGG
MboI 324 GATC ^GATC
532 GATC
MspI 20 CCGG C^CGG
30 CCGG
NciI 20 CCGGG CC^[GC]GG
29 CCCGG
NdeII 324 GATC ^GATC
532 GATC
NlaIII 390 CATG CATG^
585 CATG
NlaIV 210 GGAACC GG[ACGT]^[ACGT]CC
414 GGTACC
RsaI 50 GTAC GT^AC
415 GTAC
Sau3AI 324 GATC ^GATC
532 GATC
ScrFI 20 CCGGG CC^[ACGT]GG
29 CCCGG
TfiI 237 GAATC G^A[AT]TC
603 GATTC
TseI 142 GCTGC G^C[AT]GC
223 GCTGC
TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^
425 CAGTG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI AluI Fnu4HI HaeIII HinfI
ItaI MnlI MseI MwoI SfaNI
Tru9I Tsp509I