Restriction map for
pF1KB9635
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AvrII BalI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsgI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco47III Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI Ksp632I MamI MboII MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 252 GTCTAC GT^[AC][GT]AC
AfaI 408 GTAC GT^AC
AflIII 917 ACGTGT A^C[GA][CT]GT
Alw44I 649 GTGCAC G^TGCAC
ApaLI 649 GTGCAC G^TGCAC
AspHI 649 GTGCAC G[AT]GC[AT]^C
AvaI 129 CCCGAG C^[CT]CG[GA]G
AviII 428 TGCGCA TGC^GCA
BamHI 733 GGATCC G^GATCC
BbeI 931 GGCGCC GGCGC^C
BbrPI 916 CACGTG CAC^GTG
BfaI 208 CTAG C^TAG
BglI 676 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 524 AGATCT A^GATCT
BlpI 150 GCTGAGC GC^T[ACGT]AGC
BpmI 496 CTGGAG CTGGAG(16/14)
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaAI 916 CACGTG [CT]AC^GT[GA]
BseRI 499 GAGGAG GAGGAG(10/8)
BsiHKAI 649 GTGCAC G[AT]GC[AT]^C
BsiWI 407 CGTACG C^GTACG
BsmBI 328 CGTCTC CGTCTC(1/5)
BsmFI 200 GGGAC GGGAC(10/14)
BsoBI 129 CCCGAG C^[CT]CG[GA]G
BsrBI 843 GAGCGG CCGCTC(-3/-3)
CelII 150 GCTGAGC GC^T[ACGT]AGC
DdeI 151 CTGAG C^T[ACGT]AG
DraIII 913 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 803 CGGCCG [CT]^GGCC[GA]
EagI 803 CGGCCG C^GGCCG
EclXI 803 CGGCCG C^GGCCG
Eco52I 803 CGGCCG C^GGCCG
EcoNI 390 CCTCCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 380 CCATGG C^C[AT][AT]GG
FspI 428 TGCGCA TGC^GCA
HindIII 433 AAGCTT A^AGCTT
HinfI 4 GACTC G^A[ACGT]TC
KasI 931 GGCGCC G^GCGCC
MaeI 208 CTAG C^TAG
MaeIII 134 GTGAC ^GT[ACGT]AC
NarI 931 GGCGCC GG^CGCC
NcoI 380 CCATGG C^CATGG
NotI 802 GCGGCCGC GC^GGCCGC
PleI 4 GACTC GAGTC(4/5)
PmaCI 916 CACGTG CAC^GTG
PmlI 916 CACGTG CAC^GTG
PvuII 322 CAGCTG CAG^CTG
RsaI 408 GTAC GT^AC
SfaNI 192 GCATC GCATC(5/9)
SfoI 931 GGCGCC GGC^GCC
StyI 380 CCATGG C^C[AT][AT]GG
Tsp45I 134 GTGAC ^GT[GC]AC
XcmI 449 CCACGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 734 GATCC GGATC(4/5)
733 GGATC
AlwNI 319 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
680 CAGCGGCTG
BanII 46 GAGCCC G[GA]GC[CT]^C
177 GGGCTC
BsiEI 749 CGGTCG CG[GA][CT]^CG
803 CGGCCG
BsmAI 329 GTCTC GTCTC(1/5)
786 GTCTC
BsrFI 66 GCCGGC [GA]^CCGG[CT]
934 GCCGGC
BssHII 188 GCGCGC G^CGCGC
463 GCGCGC
BstXI 488 CCAACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCACCACGCTGG
BstYI 524 AGATCT [GA]^GATC[CT]
733 GGATCC
Cfr10I 66 GCCGGC [GA]^CCGG[CT]
934 GCCGGC
MaeII 256 ACGT A^CGT
917 ACGT
MflI 524 AGATCT [GA]^GATC[CT]
733 GGATCC
MslI 375 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
474 CATCCTCATG
NaeI 66 GCCGGC GCC^GGC
934 GCCGGC
NgoMIV 66 GCCGGC G^CCGGC
934 GCCGGC
SmlI 331 CTCAAG C^T[CT][GA]AG
781 CTCAAG
SphI 359 GCATGC GCATG^C
869 GCATGC
XhoII 524 AGATCT [GA]^GATC[CT]
733 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KspI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI SacII Sau3AI Sau96I
ScrFI SfcI TseI TspRI