Restriction map for
pF1KB4370
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AflII Alw44I Aor51HI
ApaLI AscI AvrII BbeI BbrPI
BbvCI BfrI BlnI BseAI BsiEI
BsiWI BspEI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
FseI HpaI KasI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1845 GACGTC GACGT^C
Acc65I 5328 GGTACC G^GTACC
AgeI 3019 ACCGGT A^CCGGT
Asp718I 5328 GGTACC G^GTACC
AviII 95 TGCGCA TGC^GCA
BanI 5328 GGTACC G^G[CT][GA]CC
BcgI 2788 CGAATCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3702 TGATCA T^GATCA
BglI 3071 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2131 TACGTG [CT]AC^GT[GA]
BsaWI 3019 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2564 TGTACA T^GTACA
BspDI 3790 ATCGAT AT^CGAT
BsrFI 3019 ACCGGT [GA]^CCGG[CT]
BsrGI 2564 TGTACA T^GTACA
Cfr10I 3019 ACCGGT [GA]^CCGG[CT]
ClaI 3790 ATCGAT AT^CGAT
EcoT22I 2982 ATGCAT ATGCA^T
FauI 584 CCCGC CCCGC(4/6)
FbaI 3702 TGATCA T^GATCA
FspI 95 TGCGCA TGC^GCA
HindIII 4253 AAGCTT A^AGCTT
KpnI 5328 GGTACC GGTAC^C
MfeI 4291 CAATTG C^AATTG
MunI 4291 CAATTG C^AATTG
NheI 219 GCTAGC G^CTAGC
NsiI 2982 ATGCAT ATGCA^T
PinAI 3019 ACCGGT A^CCGGT
SmaI 35 CCCGGG CCC^GGG
SphI 2006 GCATGC GCATG^C
SspBI 2564 TGTACA T^GTACA
SwaI 1568 ATTTAAAT ATTT^AAAT
XmaCI 35 CCCGGG C^CCGGG
XmaI 35 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3234 GTCTAC GT^[AC][GT]AC
4610 GTAGAC
AccII 3032 CGCG CG^CG
4331 CGCG
AocI 1674 CCTGAGG CC^T[ACGT]AGG
2356 CCTCAGG
ApaI 38 GGGCCC GGGCC^C
381 GGGCCC
AseI 2232 ATTAAT AT^TAAT
4018 ATTAAT
AsnI 2232 ATTAAT AT^TAAT
4018 ATTAAT
BciVI 5306 GGATAC GTATCC(6/5)
676 GTATCC
BglII 2424 AGATCT A^GATCT
2972 AGATCT
BlpI 1668 GCTCAGC GC^T[ACGT]AGC
3004 GCTCAGC
Bpu1102I 1668 GCTCAGC GC^T[ACGT]AGC
3004 GCTCAGC
BsmI 2263 GAATGC GAATGC(1/-1)
2807 GAATGC
BsrBI 1134 CCGCTC CCGCTC(-3/-3)
3654 CCGCTC
BsrDI 3618 CATTGC GCAATG(2/0)
2482 GCAATG
BstBI 4000 TTCGAA TT^CGAA
5000 TTCGAA
BstUI 3032 CGCG CG^CG
4331 CGCG
Bsu36I 1674 CCTGAGG CC^T[ACGT]AGG
2356 CCTCAGG
CelII 1668 GCTCAGC GC^T[ACGT]AGC
3004 GCTCAGC
DraI 1396 TTTAAA TTT^AAA
1569 TTTAAA
DsaI 1850 CCATGG C^C[GA][CT]GG
3293 CCATGG
Eco81I 1674 CCTGAGG CC^T[ACGT]AGG
2356 CCTCAGG
EcoRI 2958 GAATTC G^AATTC
5100 GAATTC
EcoRV 3354 GATATC GAT^ATC
4774 GATATC
MvnI 3032 CGCG CG^CG
4331 CGCG
NcoI 1850 CCATGG C^CATGG
3293 CCATGG
NspV 4000 TTCGAA TT^CGAA
5000 TTCGAA
PflMI 855 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3081 CCACACATTGG
PpuMI 1873 GGGACCC [GA]G^G[AT]CC[CT]
4115 AGGACCT
PshBI 2232 ATTAAT AT^TAAT
4018 ATTAAT
SfuI 4000 TTCGAA TT^CGAA
5000 TTCGAA
Van91I 855 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3081 CCACACATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
Asp700I AspEI AspHI AspI AvaI
AvaII BalI BamHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI