##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcsI        AcyI        AfaI        AflII       
AgeI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MspI        
MunI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        TfiI        Tru9I       TseI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII          93  CGCG                 CG^CG
AflIII          8  ACATGT               A^C[GA][CT]GT
AhdI            4  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI           89  AGCT                 AG^CT
AspEI           4  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI          88  GAGCTC               G[AT]GC[AT]^C
BanI          205  GGTGCC               G^G[CT][GA]CC
BanII          88  GAGCTC               G[GA]GC[CT]^C
BmyI           88  GAGCTC               G[AGT]GC[ACT]^C
BsaJI          16  CCCAGG               C^C[ACGT][ACGT]GG
BsiHKAI        88  GAGCTC               G[AT]GC[AT]^C
BsmAI          12  GTCTC                GTCTC(1/5)
BsmFI         141  GGGAC                GGGAC(10/14)
Bsp1286I       88  GAGCTC               G[AGT]GC[ACT]^C
BspLU11I        8  ACATGT               A^CATGT
BssKI          17  CCAGG                ^CC[ACGT]GG
BstEII        105  GGTCACC              G^GT[ACGT]ACC
BstNI          17  CCAGG                CC^[AT]GG
BstPI         105  GGTCACC              G^GT[ACGT]ACC
BstUI          93  CGCG                 CG^CG
Cfr13I        222  GGCCC                G^G[ACGT]CC
DpnII          96  GATC                 ^GATC
DraIII        210  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I        4  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       105  GGTCACC              G^GT[ACGT]ACC
EcoRII         17  CCAGG                ^CC[AT]GG
HphI          107  TCACC                GGTGA(8/7)
MaeIII        106  GTCAC                ^GT[ACGT]AC
MboI           96  GATC                 ^GATC
MslI          160  CACAAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           17  CCAGG                CC^[AT]GG
MvnI           93  CGCG                 CG^CG
NdeII          96  GATC                 ^GATC
NlaIV         205  GGTGCC               GG[ACGT]^[ACGT]CC
NruI           92  TCGCGA               TCG^CGA
NspI            8  ACATGT               [GA]CATG^[CT]
SacI           88  GAGCTC               GAGCT^C
Sau3AI         96  GATC                 ^GATC
Sau96I        222  GGCCC                G^G[ACGT]CC
ScrFI          17  CCAGG                CC^[ACGT]GG
SmlI          244  CTCAAG               C^T[CT][GA]AG
StuI          125  AGGCCT               AGG^CCT
Tsp45I        106  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

Cac8I          90  GCTCGC               GC[ACGT]^[ACGT]GC
              259  GCCTGC              
DdeI           22  CTGAG                C^T[ACGT]AG
              212  CTGAG               
HaeIII        126  GGCC                 GG^CC
              222  GGCC                
NlaIII          9  CATG                 CATG^
               85  CATG                
SfaNI         156  GATGC                GCATC(5/9)
               77  GCATC               
Tsp509I       100  AATT                 ^AATT
              146  AATT                
TspRI         210  CACTG                CA[GC]TG[ACGT][ACGT]^
              238  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BstF5I      FokI        MnlI